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Method for Rapid and Nondestructive Testing of Soybean Seed Viability

A non-destructive testing technology for soybean seeds, applied in germination equipment and other directions, can solve the problems of limiting testing efficiency, time-consuming and labor-intensive, etc., and achieve the effects of high testing efficiency, simple method and reliable results.

Active Publication Date: 2018-02-02
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, conventional detection methods are time-consuming and labor-intensive, which seriously limits the detection efficiency. Therefore, it is urgent to develop a non-destructive and rapid detection method for seed viability

Method used

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  • Method for Rapid and Nondestructive Testing of Soybean Seed Viability
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  • Method for Rapid and Nondestructive Testing of Soybean Seed Viability

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Embodiment 1

[0021] Embodiment 1 The method for rapid non-destructive detection of soybean seed viability

[0022] 1. Experimental materials

[0023] Three soybean varieties were randomly selected: Zhongdou 27, Zhonghuang 13 and Heihe 13, sealed in aluminum foil bags and aged in an incubator at 50°C to obtain seeds with different viability. See Table 1 for the germination rates of each gradient seed.

[0024] Table 1 The germination rate of three varieties of soybean seeds

[0025]

[0026] 2. Experimental instruments and consumables

[0027] Instruments and equipment: Weigh the seeds with a common weighing balance, and use the German Airsense PEN3 electronic nose to obtain the odor spectrum of the seeds. PEN3 has 10 metal oxide sensors corresponding to different types of gases (Table 2).

[0028] Consumables: Soybean seeds are contained in 100ml headspace vials for chromatography.

[0029] Table 2 Performance characteristics of each sensor of PEN3 electronic nose

[0030]

[00...

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Abstract

The invention provides a fast nondestructive testing method of soybean seed vitalities. Based on the principle of electronic nose, components of volatile gas generated by seeds are detected by a metal oxide sensor array; a chemical signal of the volatile gas is converted into an electrical signal; seeds of different vitalities are distinguished according to atlas of the electrical signal; and by the combination of a BP neural network, modeling of the acquired electrical signal is carried out, and vitality detection of unknown soybean seed samples is realized. By the method, distinguishing efficiency of seeds of different vitalities can reach 100%, and prediction accuracy can reach 100%. There is no need to process the soybean seeds used in the invention, and the seeds are not directly contacted during the detection process. In addition, each detection time is only 30 seconds. Nondestructive and fast testing of soybean seed vitalities can be realized. After detection by the method, the soybean seeds can be continuously stored, sowed or used for other purposes.

Description

technical field [0001] The invention relates to the measurement of seed viability, in particular to a method for rapidly and non-destructively testing the viability of soybean seeds. Background technique [0002] Seed viability refers to the vitality of the seed, which refers to the ability of the seed to germinate and grow into a seedling. Referring to the national standards of the People's Republic of China "Crop Seed Testing Regulations" and "International Seed Testing Regulations" (International SeedTesting Association. International Rules for Seed Testing. 2009), commonly used seed viability testing methods include: conventional germination method and tetrazolium staining method . The conventional germination method is the most accurate, classic and commonly used method, but it is very time-consuming. It takes about 8 days to detect the germination of soybean seeds, and 100-400 seeds will be lost for each detection. According to the "Tetrazolium Testing Handbook" (AO...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/02
CPCA01C1/025
Inventor 辛霞赵婧陈晓玲尹广鹍张金梅卢新雄
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI