Natural anti-epilepsy activity compound and uses of the natural anti-epilepsy activity compound in pharmaceutical preparations
A compound and anti-epileptic technology, applied in the field of biomedicine, can solve problems such as unknown targets, difficulty in quality control, and complex components of Qingyangshen Tablets
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Embodiment 1
[0170] Evaluation of antiepileptic effect of compound cynawilfosideA (formula VI) in vivo:
[0171] The preparation method of the compound cynawilfoside A: 10 kg of fresh Geshanxiao roots, peeled, sliced and dried to obtain 2.7kg of dry product, crushed and extracted three times with 95% ethanol percolation, each time for 2 to 3 days, combined extracts, concentrated under reduced pressure to obtain Geshanxiao the alcoholic extract, suspend the extract in water, extract with ethyl acetate, get the ethyl acetate extract, mix the sample with 100 mesh silica gel, separate with silica gel column chromatography (200-300 mesh silica gel), separate with chloroform-methanol (10:1→5:1) gradient elution to obtain components Fr1-Fr9. Fr6 was further purified repeatedly with petroleum ether-acetone and preparative HPLC to obtain pure compound cynawilfosideA (1.4 g).
[0172]
[0173] The physical and chemical data of the compound cynawilfoside A are as follows: white amorphous powder...
Embodiment 2
[0178] Evaluation of compound wilfosideC1N (formula IV) antiepileptic effect in vivo:
[0179] The preparation method of Wilfoside C1N: a. fresh 10kg of Auricularia spp., sliced and dried to obtain 3Kg dry product, pulverized and extracted three times with 95% ethanol, the extracts were combined, concentrated under reduced pressure to obtain ethanol extract, mixed with silica gel, passed 200 -300 mesh silica gel, gradient elution with petroleum ether-acetone (3:1→2:1), and then gradient elution with chloroform-methanol (10:1→5:1) to obtain fractions Fr1-Fr6. Fr3 was further purified repeatedly by petroleum ether-acetone silica gel column chromatography to obtain pure compound wilfosideC1N (1.5g); b. 10kg of fresh Geshanxiao root, peeled, sliced and dried to obtain 2.7kg of dry product, pulverized and extracted by percolation with 95% ethanol Three times, 2 to 3 days each time, combine the extracts, concentrate under reduced pressure to obtain the alcohol extract of Geshanx...
Embodiment 3
[0185] Evaluation of compound wilfosideK1N (formula V) antiepileptic effect in vivo:
[0186] The preparation method of Wilfoside K1N: a. 10kg of fresh cowhide tuber, sliced and dried to obtain 3kg dry product, crushed and extracted three times with 95% ethanol, the extracts were combined, concentrated under reduced pressure to obtain ethanol extract, mixed with silica gel, passed 200-300 Mesh silica gel, gradient elution with petroleum ether-acetone (3:1→2:1), and then gradient elution with chloroform-methanol (10:1→5:1) to obtain fractions Fr1-Fr6. Fr3 was further purified repeatedly by petroleum ether-acetone silica gel column chromatography to obtain pure compound wilfosideK1N (2.5g); b. 10kg of fresh Geshanxiao root, peeled, sliced and dried to obtain 2.7kg dry product, pulverized and extracted by percolation with 95% ethanol Three times, 2 to 3 days each time, combine the extracts, concentrate under reduced pressure to obtain the alcohol extract of Geshanxiao, suspen...
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