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Rice non-endosperm expression promoter SAFES3 and application thereof

A non-endosperm, promoter technology, applied in the field of biotechnology and crop genetic engineering, can solve the problem that research results have no application value, and achieve the effect of reducing food safety risks, improving growth performance, and helping commercial promotion.

Active Publication Date: 2016-05-11
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] If transgenic rice cannot be commercialized and used in production, no matter how good the research results are, they will have no application value

Method used

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  • Rice non-endosperm expression promoter SAFES3 and application thereof
  • Rice non-endosperm expression promoter SAFES3 and application thereof
  • Rice non-endosperm expression promoter SAFES3 and application thereof

Examples

Experimental program
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Embodiment 2

[0057] In order to verify that the sequence in SEQIDNo: 2 of the present invention also has the same function, the present invention adopts the similar scheme in Example 1, but changes the primer sequence, and conducts the same experiment as in Example 1.

[0058] It has been verified by experiments that although the transformation is lower than in Example 1, the specific expression of Gus gene in non-endosperm parts can still be induced. It was confirmed that the sequence shown in SEQ ID No: 2 has a promoter function similar to that of SEQ ID No: 1.

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Abstract

The invention provides a rice non-endosperm expression promoter SAFES3 and application thereof. The invention further provides an expression cassette and crop expression vector containing the promoter. Specifically, the invention provides application of the promoter in crop gene engineering. The promoter provided by the invention can be used for specifically driving the expression of exogenous genes in rice non-endosperm tissue, and advantages of transgenic breeding are brought into full play by using characteristics of the non-endosperm expression promoter, so as to breed more and better new varieties. Verified by experiments, the promoter provided by the invention can be used for driving the expression of the exogenous genes in non-edible (non-endosperm) parts of rice and cannot result in the expression in edible parts. Once the promoter is extensively used, popularization is very convenient, and the quality of rice can be protected from transgenic influence as much as possible, so that the elimination of people's doubt about transgenic products is better facilitated.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and crop genetic engineering. Specifically, the present invention relates to a rice non-endosperm expression promoter and its application. Endosperm structural properties for the purpose of improving rice traits. Background technique [0002] Rice is one of the most important crops, and more than one-third of the world's population uses rice as a staple food. In my country, the production area of ​​rice accounts for about 40% of the crop planting area, and rice production occupies a very important position in the national economy. Due to the constant changes in the natural environment and the needs of economic development, some practical production problems that are difficult to solve by conventional breeding techniques, such as insect pests, diseases and adversity stress, need to be solved by modern biotechnology. Transgenic technology can modify the genetic material of plants at the ge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/82C12N15/84A01H5/00
CPCC07K14/415C12N15/8205C12N15/8225C12N15/8226C12N15/8227
Inventor 秦瑞英杨剑波李莉魏鹏程李浩李娟杨亚春许蓉芳
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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