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Application of fusidic acid or pharmaceutical salt of fusidic acid in preparation of anti-HFMD (hand-foot-and-mouth disease) drug

A technology for fusidic acid and hand, foot and mouth disease is applied in the field of new use in the preparation of anti-hand, foot and mouth disease medicines, and can solve the problems of no anti-virus activity of fusidic acid and unclear anti-virus mechanism.

Inactive Publication Date: 2016-05-18
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, studies have shown that fusidic acid also has anti-HIV and JCV virus activity, but the mechanism of antiviral action is not clear
In addition, there is no report of fusidic acid's activity against other viruses

Method used

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  • Application of fusidic acid or pharmaceutical salt of fusidic acid in preparation of anti-HFMD (hand-foot-and-mouth disease) drug
  • Application of fusidic acid or pharmaceutical salt of fusidic acid in preparation of anti-HFMD (hand-foot-and-mouth disease) drug
  • Application of fusidic acid or pharmaceutical salt of fusidic acid in preparation of anti-HFMD (hand-foot-and-mouth disease) drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] EV71-MZ201405 and CVA16-MZ201509 were respectively infected into Vero or RD cells. At different drug concentrations, the inventors analyzed the expression of the reporter gene GFP carried by the virus genome by flow cytometry, and observed the cytopathic effect (CPE) caused by virus infection, As well as plaque analysis of the production of progeny viruses, it was evaluated whether sodium fusidate has an inhibitory effect on the replication of viruses EV71 and CAV16, and whether it has a protective effect on host cells.

[0024] 1.1 Cytotoxic effect of drugs: 293A-SCARB, RD and Vero cells were mixed with 1x10 4 / Each well was inoculated in 96 wells in 5% CO 2 , cultured overnight at 37°C, and treated the cells with a series of doubling-diluted fusidic acid for 48 hours, and then used the MTT method to detect the toxicity of the drug to the cells; cells cultured under the same conditions without drug treatment were used as controls; Each group of cells had three replica...

Embodiment 2

[0032] 2.1 Detection of the effect of fusidic acid addition time on antiviral activity: 5x10 4 RD cells were inoculated into 24-well plates and cultured for 18-24 hours, then infected with EV71-MZ201405 at MOI=5, and 100 μM Fussi was added 1 hour before infection (-1) and 0 hours, 1 hour, 2 hours, 4 hours, 6 hours, and 8 hours after infection. Sodium diacetate, the supernatant was collected 10h after infection, and the virus titer was determined by plaque analysis. As a control, after the virus infected the cells, DMSO was added at different time points, and the supernatant was collected 10 hours after infection and the virus titer was determined to exclude the influence of the solvent DMSO on the virus. Such as image 3 As shown, the addition of drugs from before infection to 2 hours after infection has a similar activity of inhibiting virus production, and adding sodium fusidate after 4 hours of infection has a significantly weakened inhibitory effect on virus titer, indica...

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Abstract

The invention discloses an application of fusidic acid or a pharmaceutical salt of the fusidic acid in preparation of an anti-HFMD (hand-foot-and-mouth disease) drug. The fusidic acid or the pharmaceutical salt of the fusidic acid produces fewer clinical side effects, is low in toxicity and high in safety, has activity resistant to an enterovirus EV71 and a coxsackievirus CAV16, and has potential application value in the aspect of development of a drug or a lead compound for treating HFMD.

Description

technical field [0001] The invention belongs to the field of new application of medicine. More specifically, it relates to a series of new uses of fusidic acid or its pharmaceutically acceptable salts in the preparation of anti-hand, foot and mouth disease drugs. Background technique [0002] Hand-foot-and-mouth disease (HFMD) is an acute infectious disease caused by enterovirus, mainly in children under 5 years old. The main clinical symptoms of HFMD are maculopapules and herpes on the hands, feet, mouth and other parts. Severe neurological diseases and even death may occur in severe patients. There are more than 20 enteroviruses (types) that cause HFMD, among which coxsackievirus A16 (CVA16) and enterovirus 71 (EV71) are the most common, and other different types of enteroviruses and coxsackieviruses are also Can cause disease. EV71 and CVA16 are highly contagious, and the infection is distributed globally, especially in the Asia-Pacific region. The resulting hand, foot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/56A61P31/14
CPCA61K31/56
Inventor 曾施暖郭学敏
Owner SUN YAT SEN UNIV