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Synthesizing method for fluorescent copper nano cluster

A technology of fluorescent copper nano-clusters and synthesis methods, applied in the field of biochemistry, can solve problems such as easy oxidation and difficult synthesis, and achieve the effects of low toxicity, good water solubility and high stability

Inactive Publication Date: 2016-05-25
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the synthesis of ultra-small sized copper nanoclusters is very difficult, and even the synthesized copper nanoclusters are easily oxidized in air, the preparation of ultra-small sized and highly stable copper nanoclusters is still a major challenge at present. , therefore, the synthesis of Cu nanoclusters is still at a preliminary stage compared with the synthetic methods of Au and Ag nanoclusters

Method used

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  • Synthesizing method for fluorescent copper nano cluster
  • Synthesizing method for fluorescent copper nano cluster
  • Synthesizing method for fluorescent copper nano cluster

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～5

[0022] A kind of synthetic method of fluorescent copper nano-cluster, described synthetic method is first the Cu(NO of 5mL different concentrations 3 ) 2 The solution was stirred with 5 mL of lysozyme solution with a concentration of 20 mg / mL at 25°C for 10 minutes, then 1 mL of hydroxylamine hydrochloride with a concentration of 8M was added thereto, the pH was adjusted to 12 with NaOH, and the reaction was stirred at 25°C for 16 hours to obtain fluorescent copper nanoclusters;

[0023] Described embodiment 1~5Cu(NO 3 ) 2 The concentrations of the solutions are listed in Table 1.

[0024] Table 1 Example 1~5Cu(NO 3 ) 2 concentration of solution

[0025]

Embodiment 6～12

[0027] A kind of synthetic method of fluorescence copper nanocluster, described synthetic method is that first 5mL concentration is the Cu(NO of 5mM 3 ) 2 The solution was stirred with 5 mL of lysozyme solution with a concentration of 20 mg / mL at 25°C for 10 minutes, then 1 mL of hydroxylamine hydrochloride of different concentrations was added thereto, the pH was adjusted to 12 with NaOH, and the reaction was stirred at 25°C for 16 hours to obtain fluorescent copper nanoclusters;

[0028] The concentrations of hydroxylamine hydrochloride in Examples 6-12 are shown in Table 2.

[0029] The concentration of table 2 embodiment 6~12 hydroxylamine hydrochloride

[0030]

Embodiment 13～17

[0032] A kind of synthetic method of fluorescence copper nanocluster, described synthetic method is that first 5mL concentration is the Cu(NO of 5mM 3 ) 2 The solution was stirred with 5 mL of lysozyme solution with a concentration of 20 mg / mL at 25°C for 10 minutes, and then 1 mL of hydroxylamine hydrochloride with a concentration of 8M was added thereto to adjust different pH values, and stirred at 25°C for 16 hours to obtain fluorescent copper nanoclusters;

[0033] See Table 3 for the pH values ​​of Examples 13-17.

[0034] The pH value of table 3 embodiment 13~17

[0035]

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Abstract

The invention relates to a synthesizing method for a fluorescent copper nano cluster and belongs to the field of biochemistry technologies. According to the synthesizing method, first, a solution containing Cu+2 and a lysozyme solution are mixed evenly, then hydroxylammonium chloride is added into the mixture, the pH value is adjusted to be 10-13, reaction is performed for 6-24 h, and the mole ratio of Cu+2 to lysozyme to hydroxylammonium chloride is 1:1-8:40-400. The synthesizing method has the advantages that the synthesized fluorescent copper nano cluster emits bright fluorescence and is good in water solubility, high in stability, good in biocompatibility and low in toxicity.

Description

technical field [0001] The invention relates to a method for synthesizing fluorescent copper nanoclusters, belonging to the technical field of biochemistry. Background technique [0002] In recent years, ultrasmall fluorescent metal nanoclusters containing a few to tens of metal atoms, linking atoms and large-sized nanoparticles, have attracted extensive attention due to their size-dependent unique physical and chemical properties. Since the size of metal nanoclusters is similar to the Fermi wavelength of electrons, they exhibit discrete energy levels and molecular-like properties, such as size-tunable electronic transition states and strong fluorescence properties. Metal nanoclusters are considered as novel, potential, and practical nanomaterials with practical applications in many fields, such as: cell imaging, ion sensing, and catalysis. Compared with traditional fluorescent quantum dots, metal nanoclusters have many superior properties, such as: low toxicity, ultra-smal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24B22F1/00B82Y30/00B82Y40/00
CPCB82Y30/00B82Y40/00B22F9/24B22F1/054
Inventor 韩冰雁许杰侯绪芬相荣超李莹彭婷婷于明波
Owner DALIAN UNIV OF TECH
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