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Combined evaluation model for bioavailability and toxicity of arsenic in foods on human body

A bioavailability and evaluation model technology, applied in the field of joint evaluation model, can solve the problems of lack of liver environment, differences in arsenic absorption process, differences in actual bioavailability of arsenic, etc., and achieve the effect of sensitive and efficient evaluation

Inactive Publication Date: 2016-06-01
GUANGXI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this model lacks the important link of the liver environment, which is different from the absorption process of arsenic in the human body, and leads to differences between the experimental results and the actual biological availability of arsenic

Method used

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  • Combined evaluation model for bioavailability and toxicity of arsenic in foods on human body
  • Combined evaluation model for bioavailability and toxicity of arsenic in foods on human body
  • Combined evaluation model for bioavailability and toxicity of arsenic in foods on human body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Examples of the bioavailability and toxicity of arsenic in rice and vegetables measured by the present invention to the human body

[0047] 1. Test material

[0048] 1.1 Cell lines were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences.

[0049] 1.2 Main reagents and medicines

[0050] DMEM medium, fetal bovine serum, and HBSS were purchased from Gibco,

[0051] Trypsin, pepsin, bile, non-essential amino acids were purchased from Sigma Company,

[0052] Penicillin and streptomycin were purchased from Amresco,

[0053]The rest of the reagents were of domestic analytical grade.

[0054] The buffer used is HBSS solution, pH 7.2-7.35.

[0055] 1.4 Main Equipment and Instruments

[0056] Six-well culture plate (Costar), Transwell insert cell culture dish (polyester membrane, pore size 0.4 μm), ICP-MS (PENexION300X, USA), Millicell-ERS transmembrane resistance meter, ultrapure water system (Millipore, USA) , CO 2 Incubator (Sanyo, Japan), Invert...

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Abstract

The invention provides a combined evaluation model for bioavailability and toxicity of arsenic in foods on a human body. The model comprises a Caco-2 cell model, a QSG7701 liver cell model and a container which comprises a top side chamber and a substrate side chamber which are separated by a porous membrane, wherein a Caco-2 cell is cultured in the top side chamber, and a QSG7701 liver cell is cultured in the substrate side chamber. The combined evaluation model is structurally similar to the structure of a human body intestinal absorption, can be used for simulating the absorption efficiency of a human body on arsenic in foods and sensitively and efficiently evaluating the toxicity of arsenic in foods, and can be widely applied to food safety evaluation of arsenic in foods.

Description

technical field [0001] The invention relates to a joint evaluation model of the bioavailability and toxicity of arsenic in food to human body. Background technique [0002] Arsenic is a pollutant that exists widely in nature and can cause harm to human health through the food chain. Food safety evaluation is an important guarantee for the prevention of human arsenic poisoning. At present, most evaluation methods are based on the accumulation of arsenic in food to indirectly calculate the biological effects of arsenic intake in food on the human body. This method cannot truly reflect the biological effects of arsenic intake in food on the human body. In addition, the use of animal experiments is also a method for evaluating the human bioavailability of arsenic in food, but its application is limited by relatively long experimental periods, large individual differences, and high experimental costs. Therefore, there are often deviations in toxicity evaluation. Human experimen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12N5/071C12Q1/02
CPCC12M25/02C12N5/067C12Q1/02
Inventor 韦燕燕郑小曼顾明华黎晓峰沈方科
Owner GUANGXI UNIV
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