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Application of lead-contaminated soil restoring fixing agent synthesized based on microorganism assembly

A lead-contaminated soil and fixative technology, applied in application, soil conditioning materials, fertilization devices, etc., can solve problems that are not conducive to practical application, eutrophication of water body, easy to flow away with water, etc., to achieve large-scale treatment, strong Adsorption, process cleaning and sustainable results

Inactive Publication Date: 2016-06-08
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once the nano-scale hydroxyapatite powder is dispersed in large-scale use, it is easy to flow away with the water, resulting in eutrophication of the water body
This not only leads to material loss and increases costs, but also introduces secondary pollution, which is not conducive to its practical application

Method used

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  • Application of lead-contaminated soil restoring fixing agent synthesized based on microorganism assembly
  • Application of lead-contaminated soil restoring fixing agent synthesized based on microorganism assembly
  • Application of lead-contaminated soil restoring fixing agent synthesized based on microorganism assembly

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Preparation of the present invention repair lead-contaminated soil fixative:

[0036] (1) Use PDA medium to activate and expand the cultivation of Aspergillus niger (Aspergillus niger), purchased from China Center for Type Culture Collection, strain number CCTCCAF91006;

[0037] (2) Add 1.0 g of hydroxyapatite nanoparticles (particle size97%) into 100mL of liquid PDA medium, and disperse for 0.5h with an ultrasonic wave with a power of 100 watts;

[0038](3) Put the PDA medium containing nano-hydroxyapatite into a high-temperature and high-pressure sterilizer, set at 115° C., and sterilize for 25 minutes. After high-temperature and high-pressure sterilization, the culture medium was transferred to an ultra-clean aseptic operating table, and sterilized with an ultraviolet lamp for 30 minutes;

[0039] (4) In the ultra-clean aseptic operation bench, inoculate the activated mycelium in step (1) into the sterilized medium of step (3), the inoculum size is 1 mL; the mass co...

Embodiment 2

[0043] Preparation of the present invention repair lead-contaminated soil fixative:

[0044] (1) Using improved LB medium (per 100mL medium: LB medium 2.5g, glucose 9g, sodium nitrate 0.3g) to activate and expand the culture of Penicillium Chrysogenum F1, the strain was collected in Zhuzhou City, Hunan Province, and preserved In the General Microbiology Center of the China Microbiological Culture Collection Management Committee, the preservation number: CGMCCNo.5599; a patent has been applied for.

[0045] (2) Add 1.0 g of hydroxyapatite nanoparticles (particle size 97%) into 200 mL of liquid modified LB medium, and disperse for 1.5 h with ultrasonic waves with a power of 100 watts;

[0046] (3) Put the modified LB medium containing nano-hydroxyapatite into a high-temperature and high-pressure sterilizer, set at 115° C., and sterilize for 25 minutes. After high-temperature and high-pressure sterilization, the culture medium was transferred to an ultra-clean aseptic operating...

Embodiment 3

[0051] The soil was taken from moderately polluted paddy soil around a lead-zinc tailings pond. The soil to be tested was air-dried after removing the gravel and broken roots, mixed in quarters, and crushed through a 18-mesh nylon sieve. The pH of the soil is 6.58, which is slightly acidic. The available Pb content in the soil was 479.6mg / kg.

[0052] Add 0.2g of the fixative in Example 1 (the amount of n-HAP contained in the fixative is equal to the amount of pure n-HAP weighed) and pure n-HAP into 5.0g of contaminated soil, mix well, add 10mL After 7 days of fixation, the effective state of lead in the soil was extracted with 25mL DTPA. After testing, the effective content of lead in moderately polluted soil before and after treatment with fixative or pure n-HAP is shown in Table 2 below.

[0053] Available Pb content in contaminated soil before and after treatment in Table 2

[0054]

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Abstract

The invention discloses application of a lead-contaminated soil restoring fixing agent synthesized based on microorganism assembly. Nano-hydroxyapatite serves as a central substance and wrapped with aspergillus niger hyphae, and the lead-contaminated soil restoring fixing agent is prepared, so that the problem that a powder material is excessively scattered in practical application is solved. The synthesized product is mixed with soil to be uniform according to a certain proportion, fixing is conducted for 7 days, and the available lead in the soil is reduced by 42.8%-48.6%. The fixing agent is simple in preparation process, low in cost, efficient, free of poisons, biodegradable and free of secondary contamination and is an environment-friendly fixing agent.

Description

technical field [0001] The invention relates to the technical field of combination of micro-nano material assembly and lead-contaminated soil remediation, in particular to an application method of a fixative product suitable for lead-contaminated soil remediation. Background technique [0002] Lead is a widely distributed and highly accumulative environmental pollutant in heavy metal polluted soil. Over the years, although people have taken strict control measures on the discharge of the three wastes (waste gas, waste water, waste residue), however, due to frequent human activities, with mining, metal smelting and processing, IT manufacturing, domestic sewage discharge, sewage irrigation, Lead has become one of the main elements of soil pollution due to the use of sludge and the use of leaded gasoline. Most of the lead that enters the soil exists in the topsoil layer and hardly moves downward. Lead is easy to combine with organic matter in the soil, and it is extremely dif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/40C09K101/00
CPCC09K17/40C09K2101/00
Inventor 廖骐粱丽芬邓宁熙柴立元侍维杨志辉王海鹰唐崇俭
Owner CENT SOUTH UNIV
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