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New aspergillus flavus and use thereof

A technology of Aspergillus flavus and microbial strains, which is applied to medical preparations containing active ingredients, methods based on microorganisms, plant raw materials, etc., can solve the problem of no inhibition of bacteria by Aspergillus flavus, and achieve good results in clinical application

Inactive Publication Date: 2016-06-22
GANZI TIBETAN AUTONOMOUS PREFECTURE INST OF ANIMAL HUSBANDRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no report that Aspergillus flavus and its metabolites have antibacterial activity

Method used

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  • New aspergillus flavus and use thereof
  • New aspergillus flavus and use thereof
  • New aspergillus flavus and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The isolation and identification of embodiment 1 flavus of the present invention

[0026] 1. Separation

[0027] 1.1 Conventional endophytic fungi isolation method

[0028] Cut the middle part of the leaves of healthy Ligula spp. leaves into 2mm small pieces, soak the small piece with tweezers in 75% alcohol for 30S, rinse with sterile water for 2-3 times, then immerse in 75% alcohol for 30S, rinse with sterile water for 2-3 minutes 2 to 3 times, use the last sterile water as a control, take out the small leaves with sterile tweezers and stick them flat on the PDA plate, place 6 small pieces in each plate, seal with parafilm and place it at room temperature for 5 days, then continuously observe and count. The colony shape and quantity of the control group, if no bacteria grow in the control group, it means that the surface disinfection is complete.

[0029] 1.2 Purification of endophytic fungi

[0030] Pick three typical strains and plant them on double-antibody PDA ...

Embodiment 2

[0045] Embodiment 2 adopts bacterial strain fermentation of the present invention to prepare antibacterial metabolite

[0046] 1. Experimental materials

[0047] PDA solid medium (1L): 200 grams of potatoes, 20 grams of glucose, 15 to 20 grams of agar, and the rest is water; preparation method: weigh 200 grams of potatoes, cut them into small pieces, add water and boil them (boil for 20 to 30 minutes, Just puncture the glass rod), filter with eight layers of gauze, add agar, continue to heat and mix well, after the agar is dissolved, add glucose, stir evenly, and then add water to 1000 ml after cooling down slightly.

[0048] PD liquid medium (1L): 200 grams of potatoes, 20 grams of glucose, and water for the rest; preparation method: weigh 200 grams of potatoes, cut them into small pieces, add water and boil them (boil for 20 to 30 minutes, and it can be pierced by a glass rod) ), filter with eight layers of gauze, add glucose, stir evenly, cool down slightly, and then add w...

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Abstract

The present invention discloses new aspergillus flavus and use thereof. The aspergillus flavus is aspergillus flavus LB-Y4 preserved in China General Microbiological Culture Collection Center, and the accession number is CGMCC No. 3.15413. Metabolites of the aspergillus flavus have activity for inhibition of staphylococcus aureus and other bacteria, can be used for preparing anti-bacterial drugs or disinfectants, and has good prospects for clinical application.

Description

technical field [0001] The invention relates to a new aspergillus flavus and its application. Background technique [0002] Aspergillus flavus (Aspergillus flavus), half known fungi, a common saprophytic fungus. It is more common in moldy grain, grain products and other moldy organic matter. Colony grows fast, loose structure, gray-green surface, colorless or slightly brown back. The thallus consists of many complex branched hyphae. The vegetative hyphae have separation; a part of the aerial hyphae forms long and rough conidiophores, the top of which produces a flask-shaped or subspherical apical capsule, and many small stalks (usually double-layered) are produced on the surface, and clusters are formed on the small stalks Rough-surfaced spherical conidia. Conidiophores, apical capsules, sporelets and conidia synthesize spore heads, which can be used to produce amylase, protease and phosphodiesterase, etc., and are also common strains in the brewing industry. [0003] A...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P1/02A61K36/062A61P31/04C12R1/67
Inventor 蒋忠荣胡延春罗彪刘曦洛绒志玛龙兴发叶忠明
Owner GANZI TIBETAN AUTONOMOUS PREFECTURE INST OF ANIMAL HUSBANDRY SCI
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