Day lily rooting culture method

Abstract

The invention discloses a rooting cultivation method for daylilies: cut daylily bushy seedlings multiplied in 100 ml triangular flasks into individual seedlings with an inoculation knife, and then transfer the individual seedlings to 150 ml rooting medium, each bottle Connect 5 plants, place the 150 ml Erlenmeyer flask inoculated with a single seedling into the cultivation room, adjust and control the temperature and light conditions of the cultivation room as follows: temperature 25±2°C, light intensity 2000 Lux, light time 12 h / d, and cultivate After 20 days, obtain Hemerocallis rooting seedling in 150ml triangular flask, the rooting quantity of every plant seedling is 3-5, and the length of every root is 4-7cm, and every plant height is 3-6cm; The formula is: 1 / 2 MS+IBA 0.5 mg / L+sucrose 30 g / L+agar 7 g / L, pH 5.8. After rooting culture, the appearance quality of Hemerocallis improved, and the rooting rate was greatly increased. On the 12th day of rooting culture, the rooting rate could reach 73.3%, and on the 20th day of rooting culture, the rooting rate could reach 100%.

Description

technical field [0001] The invention belongs to the application field of agricultural biotechnology, and in particular relates to a rooting culture method for daylilies. Background technique [0002] Hemerocallis is a perennial flower of the Liliaceae Hemerocallis genus. It has bright colors, graceful posture, various varieties, strong growth, and wide adaptability. It is a rare and excellent material for urban greening. At the same time, its flowers are edible and its roots can be used as medicine. If it can be widely used in landscaping, it will definitely add beauty and scenery to the city. The Hemerocallis gene is highly heterozygous, it is difficult to maintain the excellent traits of the female parent in seed reproduction, the natural ramet ability is low, the reproduction speed is slow, and the survival rate is low, so it is difficult to adapt to the requirements of commercial production. At present, the market demand is increasing day by day. The breeding of Hemeroc...

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved in the present invention is that when the industrialized cultivation of Hemerocallis is carried out by vegetative propagation by division method, there are problems such as slow propagation speed and low survival rate, which cannot meet the needs of industrialized planting, while the rooting effect of common Hemerocallis rooting medium is Not ideal, the survival rate of hardened seedlings is not high