Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of Trichoderma chrysanthemum strain and the method for using it to prepare spices for Panax notoginseng tobacco

A technology for tobacco spices and Trichoderma chrysanthemum, applied in the direction of microorganism-based methods, biochemical equipment and methods, essential oils/flavors, etc., to achieve comfortable taste, reduced bitterness, and good application prospects

Active Publication Date: 2019-04-05
CHINA TOBACCO YUNNAN IND
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no report on the use of Trichoderma chrysanthemum for the fermentation of Panax notoginseng to prepare tobacco spices

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of Trichoderma chrysanthemum strain and the method for using it to prepare spices for Panax notoginseng tobacco
  • A kind of Trichoderma chrysanthemum strain and the method for using it to prepare spices for Panax notoginseng tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Optimal conditions for enzyme production of Trichoderma chrysogreen strain HLMM-04

[0024] 1.1 Effect of initial pH of medium on enzyme production of HLMM-04 strain

[0025] The pH of the liquid medium was adjusted to 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5 respectively, and after shaking culture at 28°C and 120rpm for 3 days, the cellulase activity was measured, showing that the optimum initial pH was 6.5.

[0026] 1.2 Effect of culture temperature on enzyme production of HLMM-04 strain

[0027] Place the inoculated liquid fermentation medium at 20°C, 25°C, 28°C, 32°C, and 35°C respectively, and culture with shaking at 120r / min for 3 days, then measure the cellulase activity, which shows that the most suitable culture temperature is 28°C .

[0028] 1.3 Effect of culture time on enzyme production of HLMM-04 strain

[0029] The inoculated liquid medium (pH 6.5) was placed in a constant temperature and humidity chamber, 28°C, 120r / min shaking culture, and the cellulase a...

Embodiment 2

[0044] Repeat implementation 1, with the following differences: the amount of crude enzyme solution added is 5% of the mass of Panax notoginseng, and the fermentation time is 2h.

Embodiment 3

[0046] Repeat implementation 1, with the following differences: the amount of crude enzyme solution added is 20% of the mass of Panax notoginseng, and the fermentation time is 5 hours.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a Trichoderma chrysanthemum strain and a method for preparing spices for Panax notoginseng by using it. The strain is Trichoderma aureoviride HLMM-04 CGMCC No.10839, which has the ability to produce cellulase, and then promote the decomposition of cellulose into oligosaccharides or monosaccharides. The cellulase crude enzyme solution is obtained by activating, fermenting, filtering and dialysis of the bacterial strain, which is used to ferment Panax notoginseng, and then is extracted, filtered and concentrated under reduced pressure to make flavor for tobacco. The optimum conditions for enzyme production of HLMM‑04 strain were that the initial pH of the medium was 6.5, the culture temperature was 28°C, and the culture time was 5 days. The results of cigarette flavoring showed that the prepared spice notoginseng had obvious characteristic aroma, less bitter and astringent taste, and pleasant taste.

Description

technical field [0001] The invention belongs to the field of microbial fermentation and its application, and in particular relates to a new strain of Trichoderma chrysogreens HLMM-04, and at the same time, the invention also relates to a method for preparing spices for Panax notoginseng by using the strain. Background technique [0002] Flavoring is a key link in the cigarette process, and it is one of the effective means to effectively improve the smoking quality of cigarettes and form a unique style of cigarettes. At present, most of the tobacco flavors added to tobacco in my country are extracted by traditional methods and then blended. Although the production and operation are simple, there are also disadvantages such as monotonous aroma and ineffective improvement of cigarette smoking. The use of microorganisms to ferment aroma raw materials can increase the content of various aroma substances in traditionally extracted spices, enhance the richness of aroma, and then pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C11B9/00C12R1/885
CPCC11B9/00C12N1/145C12R2001/885
Inventor 陶鹰段焰青刘秀明党立志付磊张鹏翼董立明
Owner CHINA TOBACCO YUNNAN IND