Bacillus subtilis and its application in the control of maize spot disease
A technology for Bacillus subtilis and corn small spot disease, applied in the field of microorganisms, can solve problems such as lack of application of Bacillus subtilis, and achieve the effects of reducing the occurrence of corn small spot disease, strong antibacterial properties and strong resistance to stress.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0038]A kind of Bacillus subtilis bacterial strain of the present invention, this bacterial strain is DZSY21; Preservation name is Bacillus subtilis; Described Bacillus subtilis DZSY21 (Bacillus subtilis DZSY21) is the Eucommia plant leaf collected from the campus of Anhui Agricultural University, Hefei City, Anhui Province The tissue was obtained by using endophytic bacteria and microorganism isolation technology, and it was preserved in the General Microbiology Center (CGMCC) of the China Microbiological Culture Collection Management Committee. The preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing; preservation date: 2015 November 27; deposit number: CGMCC NO.11749.
[0039] The 16S rDNA gene sequence of the Bacillus subtilis is the nucleotide sequence shown in SEQ ID No.1, and the gyrB gene sequence of the Bacillus subtilis is the nucleotide sequence shown in SEQ ID No.2;
[0040] The single bacterium colony of described Bacillus strain is ...
Embodiment 2
[0052] The difference between embodiment 2 and embodiment 1 is:
[0053] The preparation method of bacillus subtilis bacterial agent of the present invention, comprises the steps:
[0054] In step (1), preparation of Bacillus subtilis seed liquid: Bacillus subtilis DZSY21 was inoculated in the culture solution, 50 mL of the culture solution was placed in a 250 mL conical flask, the stirring rate was 160 r / min, and cultured at 36° C. for 14 hours, to culture medium OD 600 0.9, to obtain seed liquid; the formula of the culture liquid is: 10g peptone, 5g yeast extract, 5g sodium chloride, 1L distilled water, pH 7.2.
[0055] In step (2), preparation of fermentation agent: the above-mentioned Bacillus subtilis DZSY21 seed liquid was inoculated in the fermentation liquid medium, and 350 mL of culture liquid was placed in a 1L conical flask, and the inoculation amount of the seed liquid was 2.0 %, the stirring rate is 160r / min, cultivated at 36°C for 9 hours, and adjust the cell c...
Embodiment 3
[0057] The difference between embodiment 3 and embodiment 1 is:
[0058] The preparation method of bacillus subtilis bacterial agent of the present invention, comprises the steps:
[0059] In step (1), the preparation of Bacillus subtilis seed liquid: inoculate Bacillus subtilis DZSY21 in the culture solution, place 50 mL of the culture solution in a 250 mL conical flask, stir at 160 r / min, and cultivate at 37 ° C for 16 hours, until the OD600 of the culture solution was 1.0, and the seed solution was obtained; the formula of the culture solution was: 10 g of peptone, 5 g of yeast extract, 5 g of sodium chloride, 1 L of distilled water, and pH 7.2.
[0060] In step (2), preparation of fermented bacterial agent: the above-mentioned Bacillus subtilis DZSY21 seed liquid is inoculated in the fermentation broth, 350 mL of culture liquid is placed in a 1L conical flask, and the inoculation amount of the seed liquid is 2.5 %, the stirring rate is 160r / min, cultivated at 37°C for 10 ...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More