An expression vector and its application in preparing transgenic plants

Abstract

The invention discloses an expression vector and application thereof in preparation of transgenic plants.The expression vector comprises a first expression cassette and a second expression cassette; the first expression cassette sequentially comprises the following elements of a first promoter, a selectable marker gene and a first termination sequence; the second expression cassette sequentially comprises the following elements of a second promoter, an intron and a second termination sequence; in the second expression cassette, first multiple cloning sites are arranged between the promoter and the intron, second multiple cloning sites are arranged between the intron and the termination sequence, the first multiple cloning sites and the second multiple cloning sites each has more than one enzyme digestion recognition sequence, and enzyme digestion recognition sequences located at the other positions of the expression vector are different from the enzyme digestion recognition sequences in the first multiple cloning sites and the enzyme digestion recognition sequences in the second multiple cloning sites.Experiments prove that the transgenic plants without selectable markers can be prepared through the expression vector, and the important application value on acquisition of safe transgenic plants is achieved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an expression vector and its application in preparing transgenic plants. Background technique [0002] Since the 20th century, genetic engineering breeding has been considered as an effective way to improve plant yield, quality and stress resistance. In fact, some new varieties of transgenic plants, such as transgenic soybeans, cotton, corn and rapeseed, have been bred by the use of genetic engineering breeding technology, and have been widely used in production practice. Especially in the past five years, the research on plant transgenics has developed very rapidly, and the number of plant varieties bred by transgenic methods has increased year by year. At the same time, because many national governments and consumers pay attention to the safety of genetically modified plants, as well as the inherent safety obstacles of genetically modified plants, it is difficult for most genetica...

AI Technical Summary

Problems solved by technology

Although it has been reported that transgenic tobacco, soybean, etc. without selection marker genes have been obtained by constructing double T-DNA vectors, these double T-DNA vectors are not universal due to the lack of regulatory sequences and multiple cloning sites, and each gene needs to be transformed. Constructing a double T-DNA vector from scratch through several intermediate vectors is time-consuming and laborious, and it is difficult to succeed

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