Artemia cyst shelling method
A technology of artemia eggs and shell liquid, which is applied in animal husbandry and other fields, can solve the problems of affecting the hatching rate, the hatching rate is greatly affected, and the process is unstable, so as to achieve the effect of stable shelling effect and reduce the difficulty of operation
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Embodiment 1
[0018] 1. Soak 500 grams of Artemia eggs in fresh water for 3 hours, inflate and stir to make the eggs fully absorb water and swell. If the fresh water used is tap water, it is necessary to remove chlorine in advance to reduce the damage to the shelled eggs.
[0019] 2. Separate Artemia eggs, place them in 5 liters of shelling solution, and stir at 60 r / min at a temperature of 30°C. The ingredients of the shelling liquid are: trypsin 0.8%, EDTA 0.02%.
[0020] 3. Shelling time is 2 hours, when the color of eggs gradually changes from brown to orange red, add terminator. The ingredients of the terminator are: raw soybean milk prepared from 400 grams of soybeans and 2 kilograms of water, and the soybean trypsin inhibitor in it can effectively inhibit the activity of trypsin and prevent it from destroying the egg membrane.
[0021] 4. Place the inflatable head at the bottom of the solution, open the air flow until the solution is in a tumbling state, inflate and stir for 10 min...
Embodiment 2
[0022] Embodiment 2: the impact of different shelling methods on the hatching of Artemia eggs
[0023] Using the same batch of Artemia eggs produced in Shandong, under natural light irradiation, salinity 30‰, temperature 28°C, incubation time 24h, and other conditions consistent, take the shelling rate corresponding to the two methods at different treatment times and the hatching rate of the corresponding shelled eggs, and compared the effects of two different shelling methods on the hatching of Artemia eggs, the results are shown in Table 1. Among them: the control group is normal hatching without shelling; the chemical method is the combination of sodium hypochlorite and sodium hydroxide; the hatching rate of the two shelling methods is the hatching rate of shelled eggs.
[0024] Table 1 The effect of different treatment methods on the hatching of Artemia eggs
[0025]
[0026] In the chemical method of shelling, the hatching rate of shelled eggs is negatively correlated...
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