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Primer and kit for amplifying related genes of breast cancer and ovarian cancer and application thereof

A technology for ovarian cancer and breast cancer, applied in the field of biological genes, can solve the problems of incompleteness and simplicity, and achieve the effect of easy access to samples

Inactive Publication Date: 2016-07-13
SHANDONG MEIJIA SAIPEI BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem of insufficient comprehensiveness and convenience in the analysis of key genes of breast cancer and ovarian cancer in the prior art, the present application provides primers for amplifying genes related to breast cancer and ovarian cancer, and establishes a set of primers for BRCA1, BRCA2, TP53 , PIK3CA, EGFR, KRAS, BRAF, a technique for amplifying all exons of 7 breast and ovarian cancer-related genes

Method used

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  • Primer and kit for amplifying related genes of breast cancer and ovarian cancer and application thereof
  • Primer and kit for amplifying related genes of breast cancer and ovarian cancer and application thereof
  • Primer and kit for amplifying related genes of breast cancer and ovarian cancer and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0091] 1. Isolate DNA from wax block tissue samples:

[0092] 20-40μm tumor paraffin section tissue, using the modified QIAGEN kit extraction method to separate and extract DNA, and use Nanodrop2000 micro spectrophotometer to determine the concentration and purity. The extracted DNA concentration is required to be greater than 4ng / μL, and the purity is between 1.8-2.0 (260 / 280 ratio). The extracted DNA was diluted to 4ng / μL with TEBuffer.

[0093] Concrete method is as follows (NaHSO in described step 3 , NaCl, NaOH, xylene, glycerol, and ethanol solutions are all prepared with superior pure reagents from Sinopharm):

[0094] (1) Dewaxing of paraffin specimen slices: the sample mass is 15-20 mg, put the slices in a filter column, add 200 μL of high-grade pure xylene, vortex and oscillate for dewaxing for 1 min, centrifuge, discard xylene, and add xylene again for dewaxing ;

[0095] (2) Specimen hydration: Hydrate the tissue cells with absolute ethanol, 95% ethanol, 85% et...

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Abstract

The invention relates to the technical field of biological genes and particularly relates to a primer for amplifying related genes of a breast cancer and an ovarian cancer.The primer is shown by a sequence 1-36 in a sequence table.In application of the primer and a kit in amplification of the related genes of the breast cancer and the ovarian cancer, seven related genes including a coding sequence of each gene of the breast cancer and the ovarian cancer can be simultaneously detected, and the detection saves time and is efficient.A tumor paraffin-embedded tissue slice is adopted for DNA extraction, sample obtaining is easier, and sample variety is more advantageous to achievement of accurate diagnosis.The domestic self-dependently innovated kit is developed, instead of dependency on reagents / kits of foreign companies.The detection cost is remarkably reduced, and it is expected that the detection cost is reduced by 90% compared with the commercially-available prior art.

Description

technical field [0001] The invention relates to the technical field of biological genes, in particular to a primer for amplifying genes related to breast cancer and ovarian cancer, and also to a kit and application for amplifying genes related to breast cancer and ovarian cancer. Background technique [0002] Due to factors such as environmental deterioration and population aging, the incidence of cancer continues to rise and has become the "number one killer" of human beings in the new century. In my country, cancer has become the number one cause of death among residents. It is known that genetic factors play an important role in the occurrence of tumors, as well as radiotherapy, chemotherapy and individualized treatment. On the one hand, tumorigenesis is regulated by oncogenes, and on the other hand, it is regulated by tumor suppressor genes. Regardless of surgical treatment or radiotherapy and chemotherapy, except for some patients who can achieve the expected results, ...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12N15/10C12Q1/68
CPCC12Q1/6806C12Q1/6886C12Q2600/16C12Q2531/113C12Q2537/143C12Q2565/125
Inventor 韩发彬
Owner SHANDONG MEIJIA SAIPEI BIOLOGICAL TECH CO LTD
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