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Method for biologically synthesizing nano silver through fatsia japonica leaf extract

An octagonal gold plate, biosynthesis technology, applied in the biological field, can solve the problems of limited sources of plant materials, and achieve the effect of low price, good stability and strong innovation

Active Publication Date: 2016-07-13
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to resource and geographical constraints, the sources of different plant materials are limited and may only meet the production needs of specific regions

Method used

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  • Method for biologically synthesizing nano silver through fatsia japonica leaf extract
  • Method for biologically synthesizing nano silver through fatsia japonica leaf extract
  • Method for biologically synthesizing nano silver through fatsia japonica leaf extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Biosynthesis of nano-silver particles by star anise gold plate leaf extract

[0031] a. Preparation of biological matrix of star anise gold plate leaf extract

[0032] The extracted leaves of Antonia chinensis were washed with deionized water, dried in an oven at 65°C, and then dried into powder with a pulverizer, collected for later use.

[0033] Weigh 1 g of the star anise gold plate powder, add 35 mL of deionized water, shake at 37 ° C, 180 rpm for 10 min; centrifuge at 12000 rpm for 20 min, collect the supernatant, and freeze-dry to obtain the star anise gold plate leaf extract, which is used as a matrix for nano-silver synthesis.

[0034] b. Synthesis of nano silver

[0035] Add 1 mM AgNO to the synthetic matrix at concentrations of 0, 2, 4, 6, 8 mg / mL 3 The solution was reacted for 80min under the irradiation of LED lamp (42000lx).

[0036] c. Characterization of nano silver

[0037] The synthesis of nano-silver can be characterized by color change o...

Embodiment 2

[0038] Example 2 Biosynthesis of nano-silver particles by the leaf extract of star anise gold disc

[0039] a. Preparation of biological matrix

[0040] The extracted leaves of Antonia chinensis were washed with deionized water, dried in an oven at 65°C, and then dried into powder with a pulverizer, collected for later use.

[0041] Weigh 1 g of the star anise gold plate powder, add 35 mL of deionized water, shake at 37 ° C, 180 rpm for 10 min; centrifuge at 12000 rpm for 20 min, collect the supernatant, and freeze-dry to obtain the star anise gold plate leaf extract, which is used as a matrix for nano-silver synthesis.

[0042] b. Synthesis of nano silver

[0043] Add 0.5, 1, 2, 3 mM AgNO to the synthetic matrix at a concentration of 8 mg / mL 3 , under the condition of LED lamp irradiation (42000lx), react for 80min.

[0044] c. Characterization of nano silver

[0045] After adding different concentrations of AgNO 3 Under the condition of figure 2 ), indicating the addi...

Embodiment 3

[0046] Example 3 Biosynthesis of nano-silver particles by star anise gold plate leaf extract

[0047]a. Preparation of biological matrix

[0048] Wash the extracted leaves of Antonia chinensis with deionized water, dry them in an oven at 65°C, and then use a pulverizer to make the dried leaves of Antonia chinensis into powder, and collect them for later use.

[0049] Weigh 1 g of the star anise gold plate powder, add 35 mL of deionized water, shake at 37 ° C, 180 rpm for 10 min; centrifuge at 12000 rpm for 20 min, collect the supernatant, and freeze-dry to obtain the star anise gold plate leaf extract, which is used as a matrix for nano-silver synthesis.

[0050] b. Synthesis of nano silver

[0051] Add 2mM silver nitrate solution to the synthetic matrix with a concentration of 8mg / mL, and react for 80min under the conditions of LED light irradiation (7000, 11000, 20000, 42000lx).

[0052] c. Characterization of nano silver

[0053] Such as image 3 It shows that nano-silv...

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Abstract

The invention relates to field of a biological technology, in particular to a method for biologically synthesizing nano silver through a fatsia japonica leaf extract. The method comprises the following steps: 1) washing fatsia japonica leaves for three times, drying at a temperature of 60 DEG C, grinding into power, adding super-pure water, oscillating for 10 minutes at 180 revolutions / minute, centrifuging for 20 minutes at 12000 revolutions / minute, and freezing and drying liquid supernatant to obtain the fatsia japonica leaf extract; 2) taking the fatsia japonica leaf extract obtained in the step 1) as a reaction matrix and adding a AgNo3 solution, so that the concentration of the fatsia japonica leaf extract in an obtained mixed solution is 8-10 mg / mL, and the concentration of the AgNo3 is 0.5-3 mM; reacting under the irradiation conditions of LED (Light Emitting Diode) lamps to obtain nano silver particles. The fatsia japonica leaves used in the biosynthesis method are wide in source; the method is safe and environment-friendly and high in synthesis speed, can finish the reaction within 80-100 minutes and is a green, efficient and safe biosynthesis method.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular, the invention relates to a method for biosynthesizing nano-silver through extracts from leaves of star anise aurantia discus. Background technique [0002] Nanomaterials are a research hotspot in recent years. Quantum mechanical effects at the nanoscale endow nanomaterials with various excellent properties. Nanosilver, that is, silver atoms or clusters of silver atoms with a particle size of 1-100nm, has a variety of excellent physical, chemical and optical properties, such as antibacterial properties and surface-enhanced Raman scattering effects. Nano-silver has good application potential in the fields of antibacterial materials, molecular detection and catalysts, and has attracted widespread attention of scientific researchers in recent years (see reference 1 for details: ChaloupkaK, MalamY, SeifalianAM.TrendsBiotechnol., 2010, 28: 580-588) . [0003] The traditional nano-silver sy...

Claims

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Application Information

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IPC IPC(8): C12P3/00
CPCC12P3/00
Inventor 魏雪团司果果张璟邹俊范瑞梁
Owner HUAZHONG AGRI UNIV
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