Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of BAG3 gene to preparation of bladder cancer resisting medicines

An anticancer drug, 1. BAG3 technology, applied in the direction of drug combination, antineoplastic drugs, urinary system diseases, etc., can solve the unreported expression and function of bladder cancer, affect the adhesion of tumor cells, affect the occurrence and metastasis of malignant tumors and other problems to achieve the effect of inhibiting the growth of bladder cancer cells

Inactive Publication Date: 2016-07-20
SHANXI UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

BAG3 affects the adhesion, migration and invasion of tumor cells, and then affects the occurrence and metastasis of malignant tumors in vivo, but the expression and role of BAG3 in bladder cancer have not been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of BAG3 gene to preparation of bladder cancer resisting medicines
  • Application of BAG3 gene to preparation of bladder cancer resisting medicines
  • Application of BAG3 gene to preparation of bladder cancer resisting medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: BAG3 gene overexpression lethal bladder cancer cell experiment

[0016] 1. Construction of recombinant expression vector

[0017] 1. Preparation of PCR products

[0018] Using saline-treated T24 cell cDNA as a template, PCR amplification was performed based on the full-length cDNA sequence of BAG3 gene (GeneID: 9531) and expression primers with restriction sites HindIII and BamHI were designed. The reaction conditions were: pre-denaturation at 94°C for 2 min, denaturation at 98°C for 10 s, annealing at 55°C for 30 s, extension at 68°C for 2 min, a total of 35 cycles, and incubation at 68°C for 5 min. The primer sequences are shown in Table 1. The PCR product recovery process was carried out in strict accordance with the instructions of the GelExtraction Kit of OMEGA Company.

[0019] Table 1 BAG3 gene expression primers

[0020]

[0021] 2. Full-length verification of the target gene

[0022] The target gene obtained by PCR was connected to ZeroVector ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides application of a BAG3 gene to preparation of anticancer medicines and particularly provides application of the BAG3 gene to preparation of bladder cancer resisting medicines. By construction of a BAG3 gene over-expression vector and transfection of cancer cells, according to experiments, mRNA level of the BAG3 gene in the cancer cells is raised remarkably after transfection, the cancer cells become irregular in form, round in shape and poor in wall attaching performance, activity of the cancer cells is reduced by 60.69%, and the cancer cells are dead finally. The efficient anticancer gene is obtained by screening to provide basis for discovery of new medicine targets and design of anticancer medicines.

Description

Technical field: [0001] The invention relates to a new application of BAG3 gene, in particular the application of BAG3 gene in the preparation of small molecular anticancer drugs, especially the application in the preparation of anti-bladder cancer drugs. Background technique: [0002] BCL2-associated immortality gene 3 (BAG3) is a 74kDa cytoplasmic protein, which is mainly located in rough endoplasmic reticulum, and has been identified as a BCL2-associated protein by protein interaction technology. There is a conserved domain of about 110-124 amino acids, which can bind to the ATPase domain on heat shock protein 70 (HSP70); at the same time, it can also bind to BCL2, steroid hormone receptors, Raf1, etc. In humans, the BAG family includes six members, BAG1, BAG2, BAG3, BAG4, BAG5, and BAG6. BAG3, also known as CAIR-1 or Bis, can bind different ligands through one BAG domain, including WW domain and proline repeat. As a diverse protein, BAG3 is involved in the regulation o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/711A61P35/00A61P13/10
CPCA61K31/711
Inventor 张建珍何佼张婷婷张帅娜杨喜花王建斌
Owner SHANXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com