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Method for extracting and purifying dehydrated safflower yellow B in safflower

A technology of safflower yellow pigment and purification method, which is applied in the field of extraction and purification of dehydrated safflower yellow B in safflower, and can solve the problems of few reports on the extraction process of AHSYB

Active Publication Date: 2016-07-20
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research basically focuses on hydroxysafflower yellow A, and there are few reports on the extraction process of AHSYB.

Method used

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  • Method for extracting and purifying dehydrated safflower yellow B in safflower
  • Method for extracting and purifying dehydrated safflower yellow B in safflower
  • Method for extracting and purifying dehydrated safflower yellow B in safflower

Examples

Experimental program
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Effect test

Embodiment 1

[0035] 1) Warm soaking method: put the safflower in an airtight container, add water 15 times the weight of the safflower, warm soak at 55°C for 26 minutes, filter, add the dregs to the water 9 times the weight of the original medicinal material, warm soak at 55°C 26min, filter, combine the filtrates, and concentrate under reduced pressure to become a safflower water extract with a concentration of 0.04g / mL;

[0036] 2) Pretreatment of macroporous adsorption resin: put HPD-300 macroporous resin in a beaker, first soak it in 95% ethanol for 24 hours to fully swell, then wet-pack the column, rinse with ethanol through the column until the eluate is added with water When the turbidity does not appear, wash with a large amount of water until there is no alcohol smell, and set aside;

[0037] 3) Macroporous resin adsorption: Take the safflower water extract with a concentration of 0.04g / mL, adjust the pH of the sample solution to 2.6 with dilute hydrochloric acid, pass through the ...

Embodiment 2

[0040] 1) Warm soaking method: put the safflower in an airtight container, add water 20 times the weight of the safflower, soak at 75°C for 30 minutes, filter, add the dregs to the water 11 times the weight of the original medicinal material, and soak at 75°C 26-38min, filter, combine the filtrates, concentrate under reduced pressure to become a safflower water extract with a concentration of 0.06g / mL;

[0041] 2) Pretreatment of macroporous adsorption resin: put HPD-300 macroporous resin in a beaker, first soak it in 95% ethanol for 24 hours to fully swell, then wet-pack the column, rinse with ethanol through the column until the eluate is added with water When the turbidity does not appear, wash with a large amount of water until there is no alcohol smell, and set aside;

[0042] 3) Macroporous resin adsorption: Take the safflower water extract with a concentration of 0.06g / mL, adjust the pH of the sample solution to 2.9 with dilute hydrochloric acid, pass through the HPD-30...

Embodiment 3

[0045] 1) Warm soaking method: put the safflower in an airtight container, add water 25 times the weight of the safflower, soak at 85°C for 38 minutes, filter, add water 13 times the weight of the original medicinal material to the dregs, and soak at 85°C 38min, filter, combine the filtrates, and concentrate under reduced pressure to become a safflower aqueous extract with a concentration of 0.08g / mL;

[0046] 2) Pretreatment of macroporous adsorption resin: put HPD-300 macroporous resin in a beaker, first soak it in 95% ethanol for 24 hours to fully swell, then wet-pack the column, rinse with ethanol through the column until the eluate is added with water When the turbidity does not appear, wash with a large amount of water until there is no alcohol smell, and set aside;

[0047] 3) Macroporous resin adsorption: Take the safflower water extract with a concentration of 0.08g / mL, adjust the pH of the sample solution to 3.2 with dilute hydrochloric acid, pass through the HPD-300...

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Abstract

A method for extracting and purifying dehydrated safflower yellow B in safflower belongs to the technical field of plant extracting methods and comprises the following steps: 1), extracting safflower water extract by means of warm immersion; 2), pretreating macroporous adsorbent resin; 3), adsorbing with the macroporous resin; 4), eluting with the macroporous resin. The method of the invention has high extracting rate, the content of purified dehydrated safflower yellow B is 3-4 times as high as the not purified content, and basis is provided for the efficient extraction and purification of dehydrated safflower yellow B in safflower.

Description

technical field [0001] The invention belongs to the technical field of plant extraction methods, in particular to a method for extracting and purifying dehydrated safflower yellow B in safflower. Background technique [0002] Safflower is the dried flower of Carthamustinctorius L., a plant of Compositae. It is mainly produced in Henan, Zhejiang, Sichuan and other places. It is one of the traditional Chinese medicines for promoting blood circulation and removing blood stasis, and it is also an important natural food coloring resource. Safflower yellow pigment is a mixture of various water-soluble chalcones in safflower, which has various physiological effects such as anti-oxidation, anti-thrombotic, anti-hypoxic-ischemic, hypolipidemic, analgesic, anti-inflammatory, etc. It has a good curative effect on embolism diseases, high blood pressure, diabetes complications, etc. Among the safflower yellows, hydroxyl safflower yellow A and dehydrated safflower yellow B (AHSYB) have t...

Claims

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Application Information

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IPC IPC(8): C07D405/14C09B61/00C09B67/54
CPCC07D405/14C09B61/00C09B67/0096
Inventor 何昱金湛徐森玲
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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