Bacillus subtilis fertilizer and preparation method and application thereof

A technology of Bacillus subtilis and bacterial fertilizer, applied in the field of agricultural microorganisms, can solve the problems of reduced quality of potato pieces, infection, tuber rot, etc., and achieve the effects of improving yield and quality, non-toxic side effects, and robust growth

Inactive Publication Date: 2016-07-27
山东京青农业科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] ④ Insufficient attention
At the same time, according to the different types of pathogenic bacteria, it often appears as raised or sunken surface lesions on mature potato pieces. In severe cases, the lesions are connected and the quality of potato pieces is reduced.
At the same time, since the epidermal tissue is destroyed, it is easy to be infected by other pathogenic bacteria, causing tuber rot, seriously affecting the appearance of potatoes, reducing their quality and storage period

Method used

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  • Bacillus subtilis fertilizer and preparation method and application thereof
  • Bacillus subtilis fertilizer and preparation method and application thereof
  • Bacillus subtilis fertilizer and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1: Acquisition, identification and preservation of Bacillus subtilis JQ-001 strain

[0044] 1. Obtaining of Bacillus subtilis JQ-001 strain:

[0045] The Bacillus subtilis of the present invention is obtained from the rhizosphere soil of Jiansanjiang rice in Heilongjiang Agricultural Reclamation by adopting the soil dilution separation method to separate and obtain, and the separation method is:

[0046] 1. Soil sample collection: During the normal growth period of rice, dig out the rice roots, take the soil on the roots, take several samples from one place, mix them with coarse sieves, and take about 200g for separation;

[0047] 2. Culture medium plate preparation: Melt NA medium and LB medium, pour them into sterilized petri dishes respectively, and make NA and LB plates after cooling and solidifying;

[0048] 3. Weigh 1g of soil sample into a 250ml Erlenmeyer flask with glass beads and 100ml of sterilized water, and vibrate evenly on a shaker to make 10 ...

Embodiment 2

[0059] Embodiment 2: Determination of the antagonistic effect of JQ-001 bacterial strain

[0060] Solid medium: NA medium, Gao's No. 1 medium: soluble starch 20g, KNO 3 1g, NaCl0.5g, K 2 HPO 4 0.5g, MgSO 4 0.5g, FeSO 4 0.01g agar powder 16g, distilled water 1000ml, pH7.4, when preparing, first use a small amount of cold water to make starch into a paste, pour into boiling water, heat on the fire, add other ingredients while stirring, after melting, make up When the water content reaches 1000ml, then use 100ml Erlenmeyer flasks to evenly distribute into 13 bottles, and sterilize at 121°C for 30 minutes.

[0061] Liquid medium (subtilis fermentation medium): glucose 20g, corn steep liquor powder 5g, peptone 1g, yeast powder 0.5g, MnSO 4 20ml, MgSO 4 10ml, 500ml tap water, pH7.5, sterilized at 121°C for 30min.

[0062] 1. Experimental method: plate confrontation culture method

[0063] 1. Isolation, purification and cultivation of potato scab pathogen

[0064] Sampling l...

Embodiment 3

[0076] Embodiment 3: Preparation and application of Bacillus subtilis JQ-001 microbial fertilizer

[0077] 1. Preparation of bacterial fertilizer

[0078] 1. Activation of Bacillus subtilis strains

[0079] Inoculate the strains stored in the -20°C refrigerator into NA medium test tubes, and incubate at a constant temperature of 37°C for 24 hours;

[0080] 2. Preparation of primary seeds

[0081] Prepare an appropriate amount of NA medium, divide it into two eggplant-shaped bottles, and after the high-pressure sterilization is completed, take it out and tilt it to make a slant medium, insert the activated Bacillus subtilis JQ-001, and cultivate it in a 37°C constant temperature incubator 2d;

[0082] 3. Preparation of secondary seeds

[0083] The strains cultivated in eggplant-shaped bottles are then transferred to seed tanks containing fermentation medium for cultivation. The cultivation conditions are temperature 37°C and ventilation volume 30m 3 / h, the rotation speed ...

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Abstract

The invention discloses a bacillus subtilis fertilizer and a preparation method and application thereof. The preservation number of bacillus subtilis JQ-001 is CGMCC No.11632, and the bacillus subtilis is obtained by being separated from soil of a JianSanjiang farm of Heilongjiang. The bacillus subtilis JQ-001 is subjected to seed activation, seed culture and fermented culture to be prepared into fermented liquid, then kieselguhr, a bonding agent, humic acid, cow dung and other components are added, and the bacillus subtilis fertilizer is prepared. As the bacillus subtilis has high biological activity, the inhibition capacity to potato scabs is high, environmental compatibility is good, a plant microorganism ecofauna can be adjusted, no hazard is caused to people and livestock, and use is convenient. The bacillus subtilis fertilizer prepared from the bacillus subtilis can inhibit the potato scabs, the yield of potatoes can be increased, the quality of the potatoes can be improved, the soil problem caused by potato continuous cropping all year round is solved, and intensive production development of modern agriculture is promoted.

Description

technical field [0001] The invention relates to the field of agricultural microorganisms, in particular to a Bacillus subtilis bacterial fertilizer and a preparation method and application thereof. Background technique [0002] Scab is regarded as a worldwide disease in potato production. It is common in many main potato producing areas in my country, and it is more serious in continuous cropping land, alkaline land and improper cultivation management. In recent years, scab has occurred in many potato production areas in my country, including Heilongjiang, Inner Mongolia, Hebei, Shandong, Shanxi, Shaanxi, Guizhou, Gansu, Sichuan, Hunan, Hubei, Yunnan and other major potato production areas. Reports on the disease basically covered the northeast, north, northwest and southwest of China. And the incidence is higher in areas with arid climate, alkaline soil, and severe continuous cropping. [0003] Causes of potato scab: [0004] ① Seed potatoes are infected with bacteria. T...

Claims

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Application Information

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IPC IPC(8): C05G3/00A01G13/00A01C21/00C12N1/20C12R1/125
CPCA01C21/00A01G13/00C05D9/00C05G3/00C12N1/20C12N1/205C12R2001/125C05F3/00C05F11/02C05F11/08
Inventor 史盼盼李元鑫贾振宇郭婧
Owner 山东京青农业科技有限公司
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