A kind of solid culture method of entomopathogenic nematode

Abstract

The invention discloses a solid culture method for entomopathogenic nematodes. The method is characterized by comprising the specific steps: (a) preparing an inoculum culture medium, activating symbiotic bacteria of the entomopathogenic nematodes, and inoculating the symbiotic bacteria to the inoculum culture medium; (b) inoculating a bacterial solution of the symbiotic bacteria to a solid culture medium; (c) carrying out culture for 3 to 5 days in the solid culture medium, and then, inoculating an entomopathogenic nematode suspension to the solid culture medium; (d) carrying out dark static culture on the nematodes for three weeks, thereby harvesting the nematodes. According to the method, the problem that bioculture methods cannot achieve the large-scale production of the entomopathogenic nematodes is solved, the production cost of the solid culture method is obviously lower than that of a liquid culture method, and the pathogenicity of the nematodes produced by the solid culture method is apparently higher than that of the nematodes produced by the liquid culture method. The market demand is increased, and the solid culture method has better prospects of development and has an all-important significance in the large-scale production of the entomopathogenic nematodes and the biological control on soil insects.

Description

【Technical field】 [0001] The invention relates to the technical field of biological propagation and cultivation, in particular to the technical field of solid culture methods for entomopathogenic nematodes. 【Background technique】 [0002] Subterranean pests refer to omnivorous insects that live in the soil and harm the underground parts of plants, seeds, seedlings or main stems near the soil surface during their whole life or at a certain stage of their life. Subterranean pests are the great enemies of crops. They harm seeds, young shoots, and rhizomes, resulting in lack of seedlings and even destruction of seeds, resulting in reduced crop yields, especially grubs, mole crickets, cutworms, and needleworms. [0003] At present, chemical insecticides are still the main method for underground pest control, but the effect is not ideal, and it is easy to cause environmental pollution and threaten human health and other negative effects. Controlling underground pests through high...

AI Technical Summary

Problems solved by technology

[0003] At present, the control of underground pests is still dominated by chemical insecticides, but the effect is not ideal, and it is easy to cause environmental pollution, threaten human health and other negative effects

Controlling subterranean pests through highly toxic chemical pesticides or increasing the amount of pesticide application not only leads to a substantial increase in their resistance to pesticides, but also seriously affects the quality of crops