A live propagation method of entomopathogenic nematodes

Abstract

The invention relates to a living body breeding method for entomopathogenic nematodes. The method comprises the following steps that a cultivation container is taken, a sponge block is placed into the cultivation container, and sterile water is added to the sponge block dropwisely till the sponge block is completely wetted; a proper amount of entomopathogenic nematodes water solution is dropped on the surfaces of beet armyworm larvae which are starved for 12-24 h, and the beet armyworm larvae are placed on the sponge block after 1-3 min, the cultivation container is placed into an incubator at the temperature of 22-25 DEG C for constant temperature incubation, and the sponge block is constantly in the completely-wetting state in the cultivation process; after cultivation is performed for 10-15 d, entomopathogenic nematodes are distributed in the sponge block, sterile water is used for cleaning the sponge block and the needed entomopathogenic nematodes are collected. The cultivation device is simple, the living body cultivation cost is lowered, operation is simple, host insects are wide in source and convenient to collect, the sponge block is adopted as a breeding carrier, the water driving performance of the entomopathogenic nematodes is repeatedly utilized, it is ensured that the entomopathogenic nematodes effectively infect the beet armyworm larvae, and the yield of the entomopathogenic nematodes is further ensured.

Description

technical field [0001] The invention relates to the reproduction of entomopathogenic nematodes, in particular to a method for live reproduction of entomopathogenic nematodes. Background technique [0002] Entomopathogenic nematodes are nematodes specialized in parasitic pests. They have a wide range of hosts, can actively seek hosts, are safe and non-toxic to humans, animals and the environment, and can be artificially cultivated in large quantities. They can effectively control agricultural, forestry, and horticultural plants. Boring pests, such as peach borer, various grubs, beetles, cutworms, etc., are therefore called high-potential biological control weapons by experts at home and abroad. [0003] The propagation of entomopathogenic nematodes is mainly divided into in vivo propagation and in vitro artificial medium propagation. The in vitro artificial medium propagation is further divided into solid culture and liquid culture. Both methods require a variety of raw mater...

AI Technical Summary

Problems solved by technology

[0003] The propagation of entomopathogenic nematodes is mainly divided into in vivo propagation and in vitro artificial medium propagation. The in vitro artificial medium propagation is further divided into solid culture and liquid culture. Both methods require a variety of raw materials and large-scale equipment, high investment, and operation steps Many, long production cycle, and live reproduction method is simple, few instruments and equipment are used, short production cycle, and the pathogenicity of nematodes propagated is higher than that of nematodes propagated in in vitro artificial medium

[0004] At present, the main propagation methods include White trap method, Lowtek method, liquid culture method (TSYS) and solid culture method, etc., wherein White trap method and Lowtek method generally use S. For application, utilize the method for nematode hydrotaxis to propagate pathogenic nematode, mainly have the following problems: (1) take S. wax moth as host insect, it is difficult to collect S. mellonella in the field, and the source of host insect is not wide; (2) collection method step is loaded down with trivial details, Multiple operations are required in the middle, which is time-consuming and laborious; (3) It cannot be effectively guaranteed that the parasitic insects have been effectively infected before they are placed in the breeding vessel, so that the effective reproduction in the later stage cannot be guaranteed

Both the liquid culture method and the solid culture method use artificial medium to propagate pathogenic nematodes, which mainly have the following problems: (1) animal livers are needed, and the cost is high; (2) after several generations of continuous culture, the invasion of pathogenic nematodes (3) It is necessary to cultivate the symbiotic bacteria of pathogenic nematodes, which cannot be infected by other bacteria during the reproduction process, and the operation requirements are high

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