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A strain dmc01 of I. javanica and its application in the control of East Asian migratory locusts

A technology of I. clavulata and strains, which is applied in the field of microbiology to achieve the effect of high pathogenicity and high worm rate

Active Publication Date: 2022-04-22
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the use of Isaria javanica to control East Asian migratory locusts

Method used

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  • A strain dmc01 of I. javanica and its application in the control of East Asian migratory locusts
  • A strain dmc01 of I. javanica and its application in the control of East Asian migratory locusts
  • A strain dmc01 of I. javanica and its application in the control of East Asian migratory locusts

Examples

Experimental program
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Effect test

Embodiment

[0021] 1. Isolation and purification of I. javanica strain DMC01

[0022] Collect the corpses of eucalyptus caterpillars from Qinlian Forest Farm, Qinzhou City, Guangxi Zhuang Autonomous Region, and immerse the corpses in 75% alcohol on the ultra-clean table for 30 seconds, then soak them in 0.1% mercury liter for 3 minutes, and then transfer them to Rinse with 3 bottles of sterile water, take out and place on sterilized filter paper to dissect the worms into small pieces;

[0023] Place the tissue pieces on the PDA medium with 50ug / mL streptomycin, put 5 pieces in each dish, and then culture them at 25°C. After they grow mycelium, pick the transferred strains and put them on the PDA plate The purification culture was carried out, and the purification culture was repeated 3 times. A strain of fungus obtained by screening was numbered DMC01, and the purified hyphae were picked and inserted into a PDA slant test tube. After the strain was overgrown, the slant test tube was stor...

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Abstract

The invention belongs to the technical field of microbiology, and in particular relates to a strain DMC01 of I. javanica and its application in preventing and controlling East Asian migratory locusts. A strain of Isaria javanica DMC01, whose taxonomic name is Isaria javanica, was preserved in the Guangdong Microbial Culture Collection Center on April 28, 2021, with a preservation number of GDMCC No.61632, and its gene sequence is shown in SEQ ID No. 3. The I. javanica DMC01 of the present invention has higher pathogenicity to the East Asian migratory locust, can effectively kill the East Asian migratory locust, and has a higher scorpion rate. I. javanica DMC01 of the present invention is a biological control strain with potential significance in the control of East Asian migratory locusts.

Description

technical field [0001] The invention belongs to the technical field of microbiology, and in particular relates to a strain DMC01 of I. javanica and its application in preventing and controlling East Asian migratory locusts. Background technique [0002] East Asian migratory locust Locusta migratoria manilensis (Meyen) belongs to Insecta, Orthoptera, Acrididae, alias grasshopper, locust. In China, East Asian migratory locusts are widely distributed in North China, Central China, East China, Southwest China, South China and Taiwan, and the Huanghuaihai region is a frequent occurrence area. East Asian migratory locust is a large migratory and omnivorous pest. It mainly feeds on grasses and sedges and other weeds and crops. Among them, crops eat corn, rice, sugarcane, etc., which brings great harm to agricultural production everywhere. . The formation of the East Asian migratory locust hazard is inseparable from its physiological and ecological characteristics such as fast rep...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCA01N63/30
Inventor 韦继光樊春丽江爱明谢津蒋桂和曹秀秀
Owner GUANGXI UNIV
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