A kind of Conch th130914 and its application

A technology of TH130914 and Atomyces coronatum, applied in the field of microorganisms, to achieve the effects of high spore germination rate, large spore production, and simple cultivation

Inactive Publication Date: 2016-08-24
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of auricularia coronatum TH130914 and its application, aiming to overcome the deficiency that the existing auricularia coronatum can not be found to parasitize houseflies, prevent and control flies by means of biotechnology, avoid or reduce the unreasonable use of chemical pesticides drug resistance

Method used

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  • A kind of Conch th130914 and its application
  • A kind of Conch th130914 and its application
  • A kind of Conch th130914 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment one, the isolation of pathogenic bacteria, identification

[0012] 1.1 Materials and methods

[0013] 1.1.1 Materials

[0014] The diseased housefly Boettcheriscaperegrine (Diptera: Sarcophagidae) adults were collected from Tonghai County, Yunnan Province after being infected by an entomogenic fungus.

[0015] Sabouraud medium (SDAY): 1% peptone + 1% yeast powder + 4% glucose + 1.5-2% agar powder + 1000ml water.

[0016] Sterile operating conditions: All utensils and utensils are sterilized in a high-temperature autoclave (121°C, 30min), and inoculation and other operations are performed in an ultra-clean workbench.

[0017] Culture conditions: Culture in a 25°C light (12L:12D) incubator. After the colonies are formed, transfer to the SDAY slant of the test tube, cultivate for 2 to 3 days, and transfer to a 4°C refrigerator for storage.

[0018] 1.1.2 Isolation and purification of pathogenic bacteria

[0019] Isolation: Isolation of pathogenic bacteria fr...

Embodiment 2

[0028] Embodiment two, the biological characteristics of the purified bacterial strain of Atomyces coronatum

[0029] 2.1 Materials and methods

[0030] 2.1.1 Tested strains

[0031] Select a plate with vigorous growth and uniform growth after purification as the test strain. The hyphae were inoculated on SDAY again, and cultivated in a constant temperature light (12L:12D) incubator at 25°C.

[0032] 2.1.2 Determination of colony growth rate and sporulation

[0033] Take a plate of Atomyces coronatum that has been cultivated in advance and punch holes with an 8mm hole puncher, inoculate it on another medium, do 3 repetitions, and place it in a constant temperature light (12L:12D) incubator at 25°C for cultivation. The diameter was regularly measured and recorded every day until the colony was overgrown with the culture medium. Use a puncher with a diameter of 8mm to take the bacteria cake at the same position of the culture medium, add 1% Tween-80 and 15mL SDY (Sarbourne's...

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Abstract

The present invention provides Conidiobolus coronatus TH130914 and its application. The Corynesis TH130914 was preserved in the General Microorganism Center of China Microbiology Collection Management Committee on April 8, 2014, and the preservation number is CGMCC No.9019. The invention is a bacterial strain isolated from the housefly body for the first time, and has relatively simple culture, rapid growth, large spore production and high spore germination rate. The conidia have strong pathogenicity to adults of the housefly, are environmentally friendly and pollution-free, are not easy to produce drug resistance, and can be widely used in the control of the housefly.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a ear mold TH130914 and its application. Background technique [0002] The housefly Musca domestica is an important sanitation pest. It can not only contaminate food and cause food to deteriorate, but also spread diseases through its body carrying pathogenic microorganisms. For the control of housefly, the development of insecticides has also been developed from the first generation of organochlorines to the fourth generation of pyrethroid sanitation insecticides. Although chemical insecticides have the advantages of quick effect and low cost, flies have a short life cycle (15-18 days), wide distribution, strong reproductive ability, and no diapause. If the frequency of pesticide control is too many per year, it will not only cause serious environmental pollution, but also easily kill the natural enemies of flies and make the target pests resistant to pesticid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
Inventor 肖关丽杜广祖陈斌李正跃桂富荣和淑琪严乃胜
Owner YUNNAN AGRICULTURAL UNIVERSITY
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