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A kind of I. javanica strain dmc01 and its application in the control of pine caterpillars

A technology of I. clavula and pine caterpillars, which is applied in the field of microbiology to achieve the effect of high pathogenicity and high rate of dead insects

Active Publication Date: 2022-05-20
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant report on the use of Isaria javanica to control pine caterpillars

Method used

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  • A kind of I. javanica strain dmc01 and its application in the control of pine caterpillars
  • A kind of I. javanica strain dmc01 and its application in the control of pine caterpillars
  • A kind of I. javanica strain dmc01 and its application in the control of pine caterpillars

Examples

Experimental program
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Embodiment

[0021] 1. Isolation and purification of I. javanica strain DMC01

[0022] Collect the corpses of eucalyptus caterpillars from Qinlian Forest Farm, Qinzhou City, Guangxi Zhuang Autonomous Region, and immerse the corpses in 75% alcohol on the ultra-clean table for 30 seconds, then soak them in 0.1% mercury liter for 3 minutes, and then transfer them to Rinse with 3 bottles of sterile water, take out and place on sterilized filter paper to dissect the worms into small pieces;

[0023] Place the tissue pieces on the PDA medium with 50ug / ml of streptomycin, put 5 pieces in each dish, and then culture them at 25°C. After they grow hyphae, pick the transferred strains and put them on the PDA plate The purification culture was carried out, and the purification culture was repeated 3 times. A strain of fungus obtained by screening was numbered DMC01, and the purified hyphae were picked and inserted into a PDA slant test tube. After the strain was overgrown, the slant test tube was sto...

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Abstract

The invention belongs to the technical field of microbiology, and in particular relates to a strain DMC01 of I. javanica and its application in preventing and treating pine caterpillars. A strain of Isaria javanica DMC01, whose taxonomic name is Isaria javanica, was preserved in the Guangdong Microbial Culture Collection Center on April 28, 2021, with a preservation number of GDMCC No.61632, and its gene sequence is shown in SEQ ID No.3 shown. The I. javanica DMC01 of the present invention has higher pathogenicity to pine caterpillars, can effectively kill pine caterpillars, and has a high dead worm rate. I. javanica DMC01 of the present invention is a biological control strain with potential significance in the control of pine caterpillars.

Description

technical field [0001] The invention belongs to the technical field of microbiology, and in particular relates to a strain DMC01 of I. javanica and its application in preventing and treating pine caterpillars. Background technique [0002] Pine caterpillars are the insects of the genus Pine caterpillars in the family Lepidoptera, and they are the main forest pests with a large amount of occurrence and a wide range of damage among forest pests, and they occur all the year round in various provinces of my country. According to incomplete statistics, the amount of timber growth is reduced by about 5 million cubic meters due to pine caterpillar disasters every year. In addition, when the poisonous hairs of the pine caterpillar touch the human skin, it will cause redness and swelling, and even cause joint swelling and pain, which will affect people's health. [0003] Chemical pesticides are currently the most important method to prevent pine caterpillars. However, the large-scal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCA01N63/30Y02W10/10
Inventor 韦继光樊春丽侯建敢蒋卓恩孙秋玲梁予
Owner GUANGXI UNIV
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