Composition containing obacunone and method for using obacunone in skin whitening
A technology of skin whitening and composition, applied in the field of composition including phellodendron and its use in skin whitening application, capable of solving problems such as acute dermatitis and skin irritation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0075] Embodiment 1: by in B16F10 cell (CTG) Luminescent Cell Storage Cytotoxicity / safety of Phellodendrones carried out by Luminescent Cell Viability Assay full assessment
[0076] In order to determine the cellular safety of phellodendron, the following experiment was carried out.
[0077] Should (CTG) Luminescence cell viability assay (from Promega) was performed according to the manufacturer's instructions to determine the number of surviving cells in culture based on the quantification of ATP present, an indicator of metabolically active cells. In this experiment, 99.99% pure phellodendron was used (at 100.00 μg / mL, 33.333 μg / mL, 11.111 μg / mL, 3.704 μg / mL, 1.235 μg / mL, 0.412 μg / mL, 0.137 μg / mL, 0.046 μg / mL, 0.015μg / mL) and hydroquinone (used as reference, with the same gradient concentration). This experiment was performed using B16F10 cells.
[0078] According to the cell-based safety assessment using CTG, Phellodendron showed no cytotoxicity (see Table 1)....
Embodiment 2
[0082] Embodiment 2: In B16F10 cell, the agent of enzymatic activity to tyrosinase is carried out with cortex ketone Quantity-dependent inhibition
[0083] In order to determine whether phellodendron affects the enzymatic activity of tyrosinase, the following experiment was carried out.
[0084] On day 1, B16F10 cells were plated into 24-well assay plates at a density of approximately 18,000 cells / ml. Assay plate at 37°C, 5% CO 2 , Incubate for 24 hours under humid conditions.
[0085] On the second day, the reference substance (arbutin) and the test compound (phellodine) were added (the compound on the assay plate was 5 μl (final concentration: 1×)) to the plate and the plate was incubated at 37 °C, 5% CO 2 . Incubate for an additional 2 days under humidified conditions.
[0086] On day 5, plates were imaged. The inverted plate was shaken on a medium waste receptacle and blotted with a clean paper towel. 80 μl of 0.05 mMPBS (0.1% Triton-X) was added and the plate wa...
Embodiment 3
[0098] Example 3: Phellodendrones Shows Dose-Dependent Reduction of Melanin Production
[0099] In order to determine whether phellodendron affects the production of melanin, the following experiment was carried out.
[0100] 100 ug / mL of phellodendron was tested. Arbutin, a well-known tyrosinase inhibitor, was used as a positive control inhibitor in the experiments.
[0101] Phellodendron was dissolved in DMSO and added to B16F10 cells seeded at a density of 18,000 cells / mL in 24-well plates for 24 hours prior to treatment. The final concentration of test compound is 1X. The cytosolic pH of B16F10 cells in this experiment was approximately 5-7. Each dose of phellodendron was added to the cells in triplicate. Plates were then incubated at 37 °C, 5% CO 2 , Incubate for 72 hours under humid conditions.
[0102] On day 5, the medium was removed from the cells, 150 μl of 1 M NaOH was added to each well of the assay plate and the plate was incubated at 80° C. for 30 minutes...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More