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Method assisting in identifying chickens different in pectoralis water-holding capacity and special primer of method

A hydraulic and breast muscle technology, applied in biochemical equipment and methods, microbial measurement/inspection, DNA/RNA fragments, etc., can solve problems that are difficult to apply to breeding practice, difficult to measure chicken breast meat, and large capital investment

Active Publication Date: 2016-08-03
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the water retention of chicken breast is difficult to measure in vivo, and it can only be determined by siblings or descendants.
Determination is time-consuming and requires a large capital investment, making it difficult to apply to breeding practice

Method used

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  • Method assisting in identifying chickens different in pectoralis water-holding capacity and special primer of method
  • Method assisting in identifying chickens different in pectoralis water-holding capacity and special primer of method
  • Method assisting in identifying chickens different in pectoralis water-holding capacity and special primer of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, the establishment of the method for detecting the hydraulic power of chicken breast muscle system and its effect verification

[0036] The test flocks are (120 in total): star broiler flocks (40 hens), Shouguang chicken flocks (20 hens), Beijing oil chicken flocks (20 hens), Nongda brown layer hen flocks (20 hens) and Tibetan chicken flocks (20 hens).

[0037] 1. Determination of chicken breast muscle drip loss

[0038] Detect the drip loss rate of the breast muscle of each individual in the test flock, specifically as follows:

[0039] Slaughter at the age of 140 days, take about 80-100g of the right pectoralis major muscle, weigh the weight (weight before dehydration W1), and then put the meat sample into a small net bag, put the meat sample Put the net bag into a plastic bag and fill the plastic bag with air, then tie the mouth of the bag and hang it for 24 hours (the net bag needs to be in a suspended state, so that it cannot touch the inner wall of ...

Embodiment 2

[0061] Example 2, Shouguang Chicken Hen Breast Muscle Hydraulic Power and SNP Genotype Correlation Test

[0062] The test flock is (20 in total): Shouguang chicken hens.

[0063] 1. Determination of chicken breast muscle drip loss

[0064] Detect the drip loss rate of each individual's breast muscle in the test flock respectively, the method is the same as the step one of embodiment 1.

[0065] 2. Genotyping

[0066] Detect the genotype based on the specific SNP of each individual in the test flock, as follows:

[0067] 1. Take venous blood and extract genomic DNA.

[0068] 2. Same as step 2 of embodiment 1.

[0069] The results showed that DNA fragments of about 804bp were amplified using the genomic DNA of 20 individuals as templates.

[0070] Each PCR amplification product was sequenced, all of which were 804bp. The sequencing results showed that the PCR amplification products of the 20 individuals were all shown as sequence 3 in the sequence listing. Based on the sp...

Embodiment 3

[0079] Example 3, the test of the relationship between the hydraulic power of the pectoral muscle line and the SNP genotype of the green-legged dwarf recessive white-feathered chicken

[0080] The test flock is (30 in total): green-legged dwarf recessive white-feathered roosters.

[0081] 1. Determination of chicken breast muscle drip loss

[0082] Detect the drip loss rate of each individual's breast muscle in the test flock respectively, the method is the same as the step one of embodiment 1.

[0083] 2. Genotyping

[0084] Detect the genotype based on the specific SNP of each individual in the test flock, as follows:

[0085] 1. Take venous blood and extract genomic DNA.

[0086] 2. Same as step 2 of embodiment 1.

[0087] The results showed that DNA fragments of about 804bp were amplified using the genomic DNA of 30 individuals as templates.

[0088] Each PCR amplification product was sequenced, all of which were 804bp. The sequencing results showed that the PCR ampl...

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Abstract

The invention discloses a method assisting in identifying chickens different in pectoralis water-holding capacity and a special primer of the method .According to the method assisting in identifying the chickens different in pectoralis water-holding capacity, whether genotype of the to-be-detected chicken based on specific SNP is the CC genotype or the CT genotype or the TT genotype is detected, and the pectoralis water-holding capacity of the chicken of the CT genotype is higher than that of the CC genotype; the specific SNP is as indicated in the following (a1) or (a2), wherein in (a1), DNA as indicated in sequence 3 in a sequence table in chicken genome is nucleotide at the 337 location from the 5' tail end; in (a2), the specific SNP is nucleotide at the 337 location from the 5' tail end in target sequences of a primer body F and a primer body R in the chicken genome; the primer body F is single-stranded DNA molecules as indicated in sequence 1 in the sequence table; the primer R is as indicated in the following (b3) or (b4), wherein in (b3), the primer body R is single-stranded DNA molecules as indicated in sequence 2 in the sequence table .The method and the special primer have great significance on chicken breeding and also has great significance for further researching the forming mechanism of the water-holding capacity of the chickens.

Description

technical field [0001] The invention relates to a method for assisting in identifying hydraulic chickens with different breast muscle lines and special primers thereof. Background technique [0002] With the improvement of living standards and economic development, people have higher and higher requirements for the quality and taste of chicken. my country, as a large chicken production and consumption country, is facing the embarrassing situation of the decline in meat quality brought about by the increase in meat production while the poultry industry is developing blindly in pursuit of meat production capacity. Andersen pointed out that in the process of muscle processing, water retention is one of the most important meat quality indicators. Otto and others also believe that water retention is the most important indicator of meat quality. Loss of water will bring loss of soluble protein at the same time. Savage et al. reported that there is about 112 mg of protein per mil...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 邓学梅葛雅琼秦昊高强
Owner CHINA AGRI UNIV
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