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A kind of fast return to soil cultivation method for seeds of white root

A cultivation method and technology for seeds, which are applied in the directions of seed immunity, soilless cultivation, seed and rhizome treatment, etc., can solve the problem of high production cost, reduce production cost, improve rooting rate and survival rate, and shorten aseptic cultivation time. Effect

Active Publication Date: 2018-05-01
CHENGDU JIANJIANG PHARMA FACTORY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some people have carried out research on the germination of Bletilla striata seeds and the cultivation of seedlings, such as "a method for rapid propagation and seedling cultivation of Bletilla striata seed tissue" with patent application number 201510173823. Breeding method " etc., but these methods all adopt to cultivate white and tissue culture seedling under aseptic condition, and production cost is higher

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Selection and disinfection of seeds: Select the fully mature and freshly cracked seeds of Baiji, put the seeds of Baiji and 0.1% mercuric chloride solution in a volume ratio of 1:10 into a centrifuge tube for disinfection for 11 minutes, and then use Wash with sterile water for 12 minutes;

[0019] (2) Selection and treatment of culture substrate: select the unedged paper made from old bamboo and young bamboo slurry at a ratio of 1:1 as the culture substrate, put the unedged paper into the culture tray after being sterilized at 121°C for 40 minutes, and put it in a total of 3 Layer, when put in, the rough side of the rough edge paper faces up;

[0020] (3) Germination of seeds: sow the sterilized Baiji seeds on the edging paper, and keep them with sterile water containing 20g / L sweet buckwheat germ additive (that is, every 1L of sterile water is added with 20g sweet buckwheat germ Additive) soaks the raw edge paper, and makes the seeds of Radix Rhizoma Radix germin...

Embodiment 2

[0024] (1) Selection and disinfection of seeds: select the fully ripe and freshly cracked seeds of Baiji, put the seeds of Baiji and 0.1% mercuric chloride solution in a volume ratio of 1:10 into a centrifuge tube for disinfection for 7 minutes, and then use Wash with sterile water for 8 minutes;

[0025] (2) Selection and treatment of culture substrates: Select unedged paper made from old bamboo and young bamboo slurry at a ratio of 1:1 as the culture substrate, put the unedged paper into a culture tray after being sterilized at 121°C for 30 minutes, and place a total of 2 Layer, when put in, the rough side of the rough edge paper faces up;

[0026] (3) Germination of seeds: Sow the sterilized Baiji seeds on the rough edge paper, and keep them with sterile water containing 10g / L sweet buckwheat germ additive (that is, every 1L of sterile water is added with 10g sweet buckwheat germ Additive) soaks the raw edge paper, and makes the seeds of Radix Radix Germinae germinate unde...

Embodiment 3

[0030] (1) Selection and disinfection of seeds: select the fully mature and freshly cracked seeds of Baiji, put the seeds of Baiji and 0.1% mercuric chloride solution in a volume ratio of 1:10 into a centrifuge tube for disinfection for 13 minutes, and then use Wash with sterile water for 13 minutes;

[0031] (2) Selection and treatment of culture substrates: select unedged paper made from old bamboo and young bamboo slurry at a ratio of 1:1 as the culture substrate, put the unedged paper into the culture tray after being sterilized at 121°C for 50 minutes, and place a total of 4 Layer, when put in, the rough side of the rough edge paper faces up;

[0032] (3) Germination of seeds: Sow the sterilized Baiji seeds on the rough edge paper, and keep them with sterile water containing 30g / L sweet buckwheat germ additive (that is, every 1L of sterile water is added with 30g sweet buckwheat germ Additive) soaks the raw edge paper, and makes the seeds of Radix Rhizoma Radix germinate...

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PUM

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Abstract

The invention discloses a quick backfill cultivation method of rhizoma bletillae seeds. The method comprises the following steps: selection and disinfection of seeds, selection and treatment of culture matrix, seed germination and backfill cultivation of seedlings. By adopting the method disclosed by the invention, the sterile culture time of rhizoma bletillae seedlings can be greatly shortened, the backfill cultivation can be performed within 40 days since the germination of rhizoma bletillae seeds, and thus the production cost of rhizoma bletillae seedlings can be greatly reduced. Moreover, by adopting a liquid medium with special functions, the rooting rate and survival rate of the tissue culture seedlings of rhizoma bletillae obtained by return-oil cultivation can be greatly improved.

Description

technical field [0001] The invention relates to a rapid back-to-soil cultivation method for seeds of bletilla chinensis. Background technique [0002] Baiji is a perennial herbaceous plant of Orchidaceae, which has been listed as rare and endangered plants in the country, and is also included in Appendix II of the Convention on International Trade (GITES), and is internationally protected. [0003] Baiji is not only an ornamental plant, but also an important medicinal plant. Studies have found that bletilla contains a variety of chemical components, mainly bibenzyls, phenanthrenes, dihydrophenanthrenes, flavonoids, 2-isobutylmalate glucosyloxybenzyl esters, polyphenols, etc. It has the effects of astringent and hemostasis, detumescence and muscle growth, protection of gastric mucosa, antibacterial and anticancer. [0004] At present, wild Baiji has been over-excavated, and the number has decreased sharply. And because the seeds of Baiji are very small and have no endosper...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/00A01C1/08A01G31/00A01G24/15A01G24/28A01G24/22
CPCA01C1/00A01C1/08A01G24/00A01G31/00
Inventor 王跃华刘媛媛刘曼周欢罗晴方钟若兰陈燕何新友胥华何诗红刘洪明昝加志罗倩
Owner CHENGDU JIANJIANG PHARMA FACTORY