Group of polypeptides for identification of bovine-derived ingredient in donkey-hide gelatin and products thereof

A bovine-derived, donkey-hide gelatin technology, applied in the biological field, can solve problems such as destructive properties, ineffective identification of donkey-hide gelatin, and illegal companies taking advantage of loopholes

Active Publication Date: 2016-08-10
INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Part of the reason for the repeated prohibition of donkey-hide gelatin fraud is the lack of technical support for identification. After the animal skin is boiled and dissolved, its own characteristics are destroyed, and it is difficult to identify the source of the animal skin.
At present, the country does not have an effective method to identify the ingredients of donkey-hide gelatin. It can only be controlled from the source of production. The above situation has led to many unscrupulous companies taking advantage of loopholes

Method used

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  • Group of polypeptides for identification of bovine-derived ingredient in donkey-hide gelatin and products thereof
  • Group of polypeptides for identification of bovine-derived ingredient in donkey-hide gelatin and products thereof
  • Group of polypeptides for identification of bovine-derived ingredient in donkey-hide gelatin and products thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0186] Embodiment 1, screening bovine donkey-hide gelatin collagen-specific polypeptide

[0187] Pure bovine collagen samples and donkey collagen samples were analyzed by mass spectrometry, and the mass spectrometry results were searched and compared using ProteinPilot software to determine the most preferred bovine collagen-specific peptide sequence.

[0188] First, perform mass spectrometry analysis on the selected pure collagen sample, the steps include:

[0189] (1) Sample pretreatment steps:

[0190] (1) Weigh 0.1g ~ 0.5g of pure sample homogenized into powder state, add 1% NH 4 HCO 3 solution, sonicate for 10-30 minutes to dissolve the sample completely, and dilute to 50mL;

[0191] (2) The solution is passed through a 0.22 μm filter membrane;

[0192] (3) Take 10-20 μL Trypsin enzyme solution (1 μg / μL Trypsin enzyme solution) and add it to 100 μL-200 μL of the above filtrate, enzymatically hydrolyze at 37°C for 16-18 hours, and wait for detection on the machine.

...

Embodiment 2

[0307] Example 2: Detect unspecific donkey-hide gelatin samples to determine whether it is bovine collagen bogus products

[0308] Mass spectrometry analysis was carried out on the donkey-hide gelatin boiled from animal skin and its products as well as the commercially available donkey-hide gelatin products submitted by the Public Security Bureau of a certain city to determine whether they were pure donkey collagen gelatin.

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Abstract

The invention relates to a group of polypeptides for identification of a bovine-derived ingredient in donkey-hide gelatin and products thereof, and a method using the polypeptides for identification of the bovine-derived ingredient in the donkey-hide gelatin and the products thereof through mass spectrometry,; the polypeptides in the polypeptide group has specific sequence structures and specific m/z values (including specific parent ions and a pair of daughter ions), wherein the sequences are shown in SEQ ID NO.1-50; the peptide fragments and m/z are specific to bovine-derived donkey-hide gelatin, and do not exist in donkey-derived donkey-hide gelatin.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a group of polypeptides for identifying bovine-derived components in donkey-hide gelatin and its products and a method for using the same. Background technique [0002] Donkey-hide gelatin is a solid gelatin made from the skin of an equine animal donkey, which is boiled and concentrated. It was originally produced in Dong'e County, Shandong Province, and has a history of nearly 3,000 years. Donkey-hide gelatin is a traditional top-grade nourishing and blood-enriching medicine. It tastes sweet and flat. It enters the lungs, liver, and kidneys. It has the functions of nourishing blood and stopping bleeding, nourishing yin and moistening dryness. , Anti-fatigue, improve immunity, suitable for a wide range of people. Li Shizhen's "Compendium of Materia Medica" contains: "Ejiao's "Benjing" is top grade. Hongjing said: 'It came out of Dong'e, so it is named Ejiao'". Donkey-hid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47G01N30/02G01N30/06G01N27/62G01N24/08
CPCG01N24/08G01N30/02G01N30/06G01N33/6848C07K7/08C07K7/06
Inventor 张晓梅张鸿伟鲍蕾梁成珠王境堂
Owner INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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