Thin-layer chromatography identification method of white gourd kernel formula granules

Abstract

The invention discloses a thin-layer chromatography identification method of white gourd kernel formula granules, and aims to provide a thin-layer chromatography identification method which is simple to operate and can effectively guarantee the stability of the product quality. The method is characterized by sequentially comprising the following steps of: 1) taking about 1g of the powder of the product, adding a proper amount of solvent, and performing ultrasonic processing; filtering; evaporating the filtrate to dryness; and adding methanol to the residues for dissolution to obtain the test product solution; 2) taking the control medicinal material coarse powder of the white gourd kernel; adding water, boiling and filtering; steaming the filtrate to dryness; adding a proper amount of solvent into the residues; and preparing a control medicinal material solution with the same method; and 3) conducting experiment with thin-layer chromatography; absorbing 2mul of the control medicinal material solution and 1mul of the test product solution, and dripping on the same silica gel G thin-layer plate respectively; developing with a developing solvent; taking out and airing; and spraying 0.5% ninhydrin ethanol solution, and drying until the color development of the spot is clear, wherein in the color spectrum of the test product, the same red and yellow spots are developed in the positions corresponding to the color spectrum of the control medicinal material. The method disclosed by the invention belongs to the field of traditional Chinese medicine quality detection.

Description

[0001] technical field [0002] The invention relates to a thin-layer chromatography identification method, in particular to a thin-layer identification method for winter melon kernel formula granules, and belongs to the field of traditional Chinese medicine quality inspection. Background technique [0003] Winter melon kernel formula granules are formula granules processed from the seeds of the cucurbit plant Winter Melon Benincasa hispida (Thunb.) Cogn. Its efficacy is mainly used for phlegm-heat cough, pulmonary abscess, intestinal abscess, gonorrhea, edema, beriberi, hemorrhoids, nasal and facial wine boils and other diseases. Compared with traditional Chinese medicine decoctions, it has the advantages of no need to decoct, easy to take, easy to store, convenient to carry and the like. The first part of the 2010 edition of the "Chinese Pharmacopoeia" does not include the species of winter melon kernel medicinal materials, and the traditional Chinese medicine formula gra...

AI Technical Summary

Problems solved by technology

The 2010 edition of "Chinese Pharmacopoeia" does not include the varieties of winter melon kernel medicinal materials, and the traditional Chinese medicine formula granules have lost the shape characteristics of the medicinal materials after a series of processing procedures, and the identification of properties is no longer applicable to traditional Chinese medicine formula granules.

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