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Method for rapidly breeding Spanish moss seedlings through suspension culture

A technology of suspension culture and Tillandsia, which is applied in the field of plant cultivation, can solve the problems of high cost, long cycle, and low efficiency, and achieve the effects of low cost, small footprint, and simple process flow

Active Publication Date: 2016-08-31
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cycle of this method is relatively long, and it takes 2-3 years from material collection to batch emergence. During the breeding process, relatively large manpower and material resources need to be invested in multiple batch subcultures to obtain batch seedlings, which is low in efficiency and high in cost.

Method used

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  • Method for rapidly breeding Spanish moss seedlings through suspension culture
  • Method for rapidly breeding Spanish moss seedlings through suspension culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Callus induction and proliferation

[0028] Get Tillandsia aseptic seedling leaves, cut into 50 sections of 0.5-1cm, inoculate in callus induction medium (MS+6-BA 2.0mg / L+NAA 0.02mg / L+sucrose 30g / L+carrageenan 6.5g / L, pH 5.8), after 30 days of culture, small protrusions appeared at the base of the inoculated explants, and after 40-50 days of culture, fragile callus gradually formed; after the callus was separated from the leaves, fresh callus was inoculated The culture was continued on the tissue induction medium, subcultured once every 40 days, the callus proliferated and differentiated, and a large number of light yellow, loose and fragile embryogenic calli were formed. Culture conditions: culture temperature 25°C, light culture. Lighting time 10h / d, light intensity 23μmol·m -2 ·s -1 .

[0029] 2. Liquid suspension of embryogenic callus

[0030] Gently crush the loose and friable embryogenic callus into 40 small pieces of about 2mm×2mm, and inoculate them int...

Embodiment 2

[0043] 1. Callus induction and proliferation

[0044] Get Tillandsia aseptic seedling leaves, cut into 50 segments of 0.5-1cm, inoculate in callus induction medium (MS+6-BA 2.5mg / L+NAA 0.02mg / L+sucrose 30g / L+carrageenan 6.5g / L, pH 5.8), after 30 days of culture, small protrusions appeared at the base of the inoculated explants, and after 40-50 days of culture, fragile callus gradually formed; after the callus was separated from the leaves, fresh callus was inoculated The culture was continued on the tissue induction medium, subcultured once every 40 days, the callus proliferated and differentiated, and a large number of light yellow, loose and fragile embryogenic calli were formed. Culture conditions: culture temperature 27 ℃, light culture. Lighting time 12h / d, light intensity 27μmol m -2 ·s -1 .

[0045] 2. Liquid suspension of embryogenic callus

[0046] Gently crush the loose and friable embryogenic callus into 40 small pieces of about 2mm×2mm, and inoculate them int...

Embodiment 3

[0059] 1. Callus induction and proliferation

[0060] Get Tillandsia aseptic seedling leaves, cut into 50 sections of 0.5-1cm, inoculate in callus induction medium (MS+6-BA 3.0mg / L+NAA 0.02mg / L+sucrose 30g / L+carrageenan 6.5g / L, pH 5.8), after 30 days of culture, small protrusions appeared at the base of the inoculated explants, and after 40-50 days of culture, fragile callus gradually formed; after the callus was separated from the leaves, fresh callus was inoculated The culture was continued on the tissue induction medium, subcultured once every 40 days, the callus proliferated and differentiated, and a large number of light yellow, loose and fragile embryogenic calli were formed. Culture conditions: culture temperature 29°C, light culture. Illumination time 10h / d, light intensity 30μmol·m -2 ·s -1 .

[0061] 2. Liquid suspension of embryogenic callus

[0062] Gently crush the loose and friable embryogenic callus into 40 small pieces of about 2mm×2mm, and inoculate them...

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Abstract

The invention belongs to the technical field of plant cultivation, and relates to a method for rapidly breeding Spanish moss seedlings through suspension culture. The method comprises the processes of callus induction and propagation, embryogenic callus liquid suspension, embryoid adventitious bud germination, adventitious bud root induction, transplanting and management. The method has simple process flow, and allows a large amount of seedlings used for production and cultivation to be obtained in a short time through tissue culture adopting Spanish moss leaves as a material, the early stage culture period is short, the obtained seedlings have the advantages of consistent physiologic age, consistent growth, small occupied area and low cost, and the method is suitable for factory seedling growth and large scale cultivation, and provides technical support for development of the artificial plantation industry of Spanish moss.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and relates to a method for propagating Tillandsia seedlings, in particular to a callus induced by Tillandsia leaves to induce embryogenic callus and embryoid bodies, and to culture embryoids by suspension culture. Proliferation, and then through embryoid body germination, adventitious bud growth, adventitious bud rooting induction, aseptic seedling transplanting and other processes, to obtain a large number of seedling breeding methods. Background technique [0002] Tillandsia cyanea, also known as fan pineapple, belongs to Bromeliaceae (Bromeliaceae) Tillandsia genus, is a perennial monocotyledonous plant, native to tropical America and tropical Asia, there are about 500 native species, horticultural cultivation and ornamental 60 kinds. Tillandsia has short stems and relatively short plants. The involucral bracts can be viewed for several months. It is a miniature ornamental plant wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 李志英徐立符运柳李克烈黄碧兰
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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