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Preparation method of biological matrix gel material for promoting regeneration and repair of cartilage, gel performance determination method and application

A bio-matrix and gel material technology, applied in the field of preparation of bio-matrix gel materials, achieves the effect of low price, simple materials, and promotion of cartilage damage repair

Active Publication Date: 2016-08-31
SHANDONG JINGYUAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, due to the excessive use of joints caused by aging or sports, there are more and more patients with knee articular cartilage damage, which seriously affects the quality of life of patients. Therefore, the preparation method of bio-matrix materials for promoting cartilage regeneration and repair is an important biological Method, in the existing preparation methods of biomatrix materials used to promote cartilage regeneration and repair, the treatment of osteoarthritis mainly focuses on eliminating the pain or inflammation of the affected part, which is achieved by administering non-steroidal anti-inflammatory drugs at the patient part, For elderly patients with reduced renal function, it is difficult to continuously orally administer non-steroidal anti-inflammatory drugs from the perspective of safety; it is also possible to prepare products obtained by making hyaluronic acid, one of the components of cartilage joint fluid, into the joint cavity, It can improve the lubricating function of joints and suppress pain. However, for severe osteoarthritis caused by further progression of cartilage damage, artificial joint replacement must be performed for treatment. There is no bio-matrix gel for promoting cartilage regeneration and repair. Material preparation method, gel performance measurement method and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0046] The first step, the preparation of human amniotic membrane-PEI system:

[0047] (a) Preparation of fully dissolved and decellularized human amniotic membrane: the human amniotic membrane was treated with PBS to remove blood stains, and then washed repeatedly with normal saline, the human amniotic membrane was cut into 1.8cm × 2.8cm, added 0.02% EDTA solution and shaken Mix to obtain the intermediate mixed liquid I, separate the intermediate mixed liquid by centrifugation, discard the supernatant, add 0.23 mol acetic acid solution, shake slowly in water at a temperature of 90 °C until the intermediate mixed liquid II is completely dissolved, set The parameter is 3800rpm, time 28min to centrifuge the intermediate mixed liquid Ⅱ, extract the supernatant, and put the extracted supernatant through a disinfection treatment with a pressure of 65.55KPa, a temperature of 110.6°C and a time of 8min to obtain the intermediate mixed liquid Ⅲ , store the intermediate mixed liquid II...

Embodiment 1

[0130] The preparation of embodiment 1 chitosan / human amniotic membrane-PEI / GP system

[0131] 1) Preparation and disinfection of chitosan solution

[0132] Accurately weigh 0.2g of chitosan powder with a degree of deacetylation greater than 90% and disperse it in 30ml of 0.5mol / L acetic acid solution, stir magnetically until it is completely dissolved, then dilute to 100ml with acetic acid solution to obtain a 2mg / ml shell Polysaccharide solution, stored at room temperature. Seal the evenly stirred chitosan solution with kraft paper and sterilize it in a high temperature and high pressure sterilizer at 121°C for 10 minutes. After the temperature of the sterilized chitosan solution drops to room temperature, put it in a 4°C refrigerator for storage.

[0133] 2) Preparation and disinfection of disodium glycerophosphate (GP)

[0134] The GP powder was dissolved in triple distilled water to obtain a solution with a mass concentration of 56%, sterilized by filtration through a 0...

Embodiment 2

[0137] Embodiment 2 chitosan / human amniotic membrane-PEI / GP system gel performance measurement

[0138] The gel time is determined by the inversion method: the gel time is defined as the time required to change from sol to gel, when the system is in a flowing liquid state, it is defined as a sol state, and when the system is in a non-flowing semi-solid state, it is defined as a gel state . Take 2.0ml of sol and put it in an ampoule bottle, place it in a water bath at 37°C, observe the fluidity by inverting it every 1 minute, and take the time when the sol does not flow after inverting for 30 seconds as the gel time. The results show that under this condition, the final gel temperature of the material of the present invention can be shortened to 2.5 minutes.

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Abstract

The invention provides a preparation method of a biological matrix gel material for promoting regeneration and repair of cartilage, a gel performance determination method and application. The steps include: adding a GP solution into a mixed solution of a human amniotic membrane-PEI system and a mixed solution of a chitosan solution to conduct blending treatment to obtain a chitosan / human amniotic membrane-PEI / GP system mixed solution with the biological matrix gel material as the matrix. The gel formed by the chitosan solution and the GP solution maintains the activity of cells in the mixed solution of the human amniotic membrane-PEI system, promotes the regeneration and repair of cartilage, and artificial joint replacement is no longer carried out for treatment, therefore, the non-surgical treatment effect of severe osteoarthritis formed by further development of cartilage injury is improved.

Description

1. Technical field [0001] The invention relates to a preparation method of a biomatrix gel material, in particular to a preparation method of a biomatrix gel material for promoting cartilage regeneration and repair, a method for measuring gel performance and an application thereof. 2. Background technology [0002] Due to the absence of blood vessels and nerve network, knee cartilage has almost no self-repair ability, especially when a large injury site is formed, it will cause joint pain and loss of joint function, and often develop into osteoarthritis. In addition, due to the excessive use of joints caused by aging or sports, there are more and more patients with knee articular cartilage damage, which seriously affects the quality of life of patients. Therefore, the preparation method of bio-matrix materials for promoting cartilage regeneration and repair is an important biological Method, in the existing preparation methods of biomatrix materials used to promote cartilage...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/20A61L27/38A61L27/52
CPCA61L27/20A61L27/3604A61L27/3654A61L27/3683A61L27/3834A61L27/3852A61L27/52A61L2400/06A61L2430/10C08L5/08
Inventor 田丽娜罗光会梁峰朱麟杨继建
Owner SHANDONG JINGYUAN BIOTECH CO LTD
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