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Sheep UCP1 (uncoupling protein 1) allelotype detection method and detection kit

A technology for detection kits and alleles, which can be used in the determination/inspection of microorganisms, biochemical equipment and methods, etc., and can solve the problems of not many and less research on the fat content of sheep carcasses.

Inactive Publication Date: 2016-09-07
COLD & ARID REGIONS ENVIRONMENTAL & ENG RES INST CHINESE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Previous studies on sheep UCP1 gene mainly focused on the study of tissue expression characteristics, but there are not many studies on UCP1 on carcass fat deposition, and there are fewer studies on UCP1 gene on sheep carcass fat content

Method used

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  • Sheep UCP1 (uncoupling protein 1) allelotype detection method and detection kit
  • Sheep UCP1 (uncoupling protein 1) allelotype detection method and detection kit
  • Sheep UCP1 (uncoupling protein 1) allelotype detection method and detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 A PCR-SSCP kit for the detection of sheep carcass fat content traits

[0046] Test kit of the present invention comprises:

[0047] 1. Specific primer pair:

[0048] Upstream primer: 5'-AGATACAAGCGGAAGAGACAC-3';

[0049] Downstream primer: 5'-TGAAGGGTTGGGTCTGTCA-3';

[0050] 2. Biochemical reagents:

[0051] TaqDNA polymerase; Sterile ultrapure water;

[0052] Contains MgCl 2 , 5×Buffer of dNTPs or

[0053] 10×PCR buffer, MgCl 2 and dNTPs.

[0054] Precautions:

[0055] (1) The extraction of DNA can be performed according to the requirements of whole blood DNA extraction kits from different manufacturers, but the purity of the DNA must be ensured. If the extraction effect is not good, subsequent tests need to be performed after purification;

[0056] (2) The reaction system and reaction procedures can be operated according to the product requirements of different manufacturers, but the specificity of the amplification and the concentration of the amp...

Embodiment 2

[0057] Example 2 Method for Screening DNA Molecular Markers Significantly Related to Intramuscular Fat Traits in Local Sheep Populations

[0058] In the present invention, primers are firstly designed according to the sheep gene sequence published by NCBI (GenBank Accession No. JN604985.1), and the sheep genome DNA is used as a template to carry out PCR amplification, and the amplified products are analyzed by SSCP polyacrylamide gel electrophoresis and sequencing It was found that there were polymorphisms and different allelic types in the promoter region of sheep UCP1 gene; secondly, PCR-SSCP detection of allelic types was carried out in the tested population; finally, according to the allelic types detected in the population, Statistical analysis of population genetics and association analysis of carcass fat content traits were carried out, and molecular markers closely related to intramuscular fat traits of sheep were screened out.

[0059] A molecular marker method for br...

Embodiment 3

[0110] Example 3 Establishment of Sheep Population with Quality Carcass Fat Content Traits

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Abstract

The invention provides a sheep UCP1 (uncoupling protein 1) allelotype detection kit which comprises a specific primer pair and sheep UCP1 allelotype standard DNAs, wherein the specific primer pair comprises a sense primer 5'-AGATACAAGCGGAAGAGACAC-3' and an antisense primer 5'-TGAAGGGTTGGGTCTGTCA-3'; the sheep UCP1 allelotype standard DNAs are SEQ ID No. 1-3 in a sequence table. The invention further provides a sheep UCP1 allelotype detection method. By the detection method, different sheep UCP1 allelotypes and sheep carcass fat content characters are associatively analyzed, and researches show that a sheep UCP1 allelotype has remarkable influence (P<0.05) on a carcass fat content character of a sheep population. Therefore, a sheep UCP1 gene can be determined to improve the sheep carcass fat content character as a molecular marker.

Description

technical field [0001] The invention relates to the detection and identification of gene alleles, in particular to a method for detecting alleles of sheep UCP1 and a detection kit thereof. Background technique [0002] my country is a big sheep-raising country, and the amount of sheep breeding ranks first in the world. With the changes in the world’s sheep-raising pattern in recent years, mutton sheep production has gradually become the mainstream. Demand for quality lamb products. Accelerating the improvement of sheep meat traits and improving the quality of mutton products has become an urgent problem to be solved in sheep breeding. Fat content is one of the key factors affecting the tenderness of meat. The fat characteristics of meat include carcass fat content, backfat thickness, suet percentage, flower oil percentage, IMF and intermuscular fat. Carcass fat content is the main phenotypic indicator for studying the effect of fat on meat tenderness. [0003] Carcass mea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2531/113C12Q2523/113C12Q2565/125
Inventor 杨果段子渊赵凯张玉宝王小琪谢忠奎
Owner COLD & ARID REGIONS ENVIRONMENTAL & ENG RES INST CHINESE
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