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Co-segregation molecular marker tcr541, primers and application of Chinese cabbage clubroot resistance crb gene

A TCR541, anti-clubroot disease technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as chain burden, and achieve the effect of shortening the breeding process and improving the breeding efficiency

Active Publication Date: 2019-07-05
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these markers are not markers that co-segregate with CRb, and the use of these markers for molecular marker-assisted breeding is likely to cause linkage drag

Method used

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  • Co-segregation molecular marker tcr541, primers and application of Chinese cabbage clubroot resistance crb gene
  • Co-segregation molecular marker tcr541, primers and application of Chinese cabbage clubroot resistance crb gene
  • Co-segregation molecular marker tcr541, primers and application of Chinese cabbage clubroot resistance crb gene

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Experimental program
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Embodiment Construction

[0025] The present invention will be described in detail below with reference to the drawings and specific embodiments, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments.

[0026] 1. Acquisition of co-segregation molecular markers TCR541 and TCR540 of Chinese cabbage clubroot resistance CRb gene

[0027] 1. Construction of isolated groups

[0028] F 1 , the obtained F 1 All the offspring plants showed resistance to the disease. The above two parents 'CRBJN3-2' and 'BJN3-2' are preserved in Shenyang Agricultural University. Select individual plant F 1 selfing build F 2 There are 4,783 groups in the mapping group. All 4783 Fs Seeded 2 Groups were inoculated with Plasmodium 10 days after planting. After 40 days for disease resistance identification, 4783 F 2 In the population, 3641 were resistant and 1142 were susceptible, and the chi-square test met the segregation ratio of 3:1

[0029] 2. DNA ex...

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Abstract

The invention discloses a co-segregation molecular marker TCR541 of a Chinese cabbage clubroot resisting CRb gene. The molecular marker TCR541 and the CRb gene are in co-segregation, so that the molecular marker TCR541 can be used for assistant selection of the molecular marker of the clubroot resisting gene of brassica such as Chinese cabbage and Shanghai Pak Choi. The markers can be used in the assistant selection process, the theoretical selection accuracy can reach 100%, and the marker is good in repeatability, high in reliability, low in detection cost, time-saving and labor-saving.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a co-separation molecular marker TCR541 of Chinese cabbage clubroot resistance CRb gene, primers and applications. Background technique [0002] In 2002, our research group obtained a Chinese cabbage double haploid line 'CR Shinki DH' resistant to brassica clubroot, and the research showed that the disease-resistant material was resistant to physiological races 2, 4 and 8 of Plasmodium brassicae (Piao et al., Conversion of AFLP marker linked to clubroot resistance gene into SCAR marker. 2002, J Kor Soc Hort Sci 43:653-665). Further studies have proved that the clubroot resistance gene is a dominant single gene and named as CRb (Piao et al., SCAR and CAPS mapping of CRb, a gene conferring resistance to Plasmodiophora brassicae in Chinese cabbage (Brassica rapa ssp.pekinensis), Theor Appl Genet, 2004, 108:1458-1465). [0003] During the transformation of brassica species ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/158
Inventor 朴钟云张腾庞文星李晓楠朴英兰张椿雨
Owner SHENYANG AGRI UNIV