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Stress-resistant near-isogenic lines of parthenogenetic insects and their construction methods and applications

A near-isogenic, parthenogenetic technology, applied in animal husbandry and other directions, to achieve the effect of simple steps, easy operation, and elimination of differences in genetic backgrounds

Active Publication Date: 2018-08-31
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

How to establish drug-resistant near-isogenic lines of parthenogenetic insects is still a blank in this field

Method used

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  • Stress-resistant near-isogenic lines of parthenogenetic insects and their construction methods and applications
  • Stress-resistant near-isogenic lines of parthenogenetic insects and their construction methods and applications
  • Stress-resistant near-isogenic lines of parthenogenetic insects and their construction methods and applications

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Experimental program
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Embodiment 1

[0028] Embodiment 1. Method for constructing parthenogenetic insect stress-resistant near-isogenic lines of the present invention and constructed near-isogenic lines

[0029] This embodiment provides a method for constructing a stress-resistant near-isogenic line of parthenogenetic insects, comprising the following steps:

[0030] (1) Selecting the female adults of the stress-sensitive strain to hybridize with the male adults of the stress-resistant strain to obtain 1 generation of hybridization;

[0031] (2) selecting female adults from the first generation of hybridization, and making them parthenogenetic to obtain a single male offspring;

[0032] (3) screen the single male offspring with the stress, collect the surviving male adults, and repeat the hybridization described in step (1) with the female adults of the sensitive strain;

[0033] Taking the above (1)-(3) as a cycle, carry out multiple rounds of hybridization cycles;

[0034] The hybrid progeny female adults obt...

Embodiment 2

[0037] Embodiment 2, adopt the method of the present invention to construct western flower thrips parthenogenetic insect insecticide-resistant near isogenic line

[0038] (1) Using the method described in Example 1, the sensitive strain (Ivf03) of Western flower thrips that has been raised indoors for many years and the resistant strain (Spin_R) that is derived from Ivf03 and obtained by selection with spinosyn as test insect materials , for the construction of spinosyn-resistant near-isogenic lines of Thrips occidentalis.

[0039](2) Preparation of parental materials: Pick a single pupae from a sensitive strain at the pupal stage and place it in a 2mL centrifuge tube. All obtained in this way) were crossed with males of the resistant strain (genotype r♂) to obtain F1 offspring (containing heterozygous sensitive female Sr♀ and sensitive male r♂).

[0040] (3) When the F1 generation developed to the pupal stage, single-headed pupae were picked and placed in 2 mL centrifuge tub...

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Abstract

The invention provides a method for establishing an anti-adversity near-isogenic line of parthenogenesis insects and belongs to the technical field of insect inheritance. The method comprises steps as follows: (1) an adversity-sensitive line female adult insect and an adversity-resistant line male adult insect are selected for hybridization, and a first filial generation is obtained; (2) female adult insects are selected from the first filial generation and subjected to parthenogenesis, and single male progeny are obtained; (3) the single male progeny are treated and cultivated under adversity, survival male adult insects are screened, collected and hybridized, like hybridization in the step (1), with the sensitive line female adult insect, the steps from (1) to (3) form a cycle, multiple hybridization cycles are performed, the female adult insects of hybrids obtained through the final hybridization are backcrossed with the male parent, obtained offspring are treated and cultivated and screened under adversity, and then the survival offspring belong to the anti-adversity near-isogenic line of the parthenogenesis insects. The method has the advantages of being easy to operate, simple in step, convenient to implement and the like, and the cost is lower.

Description

technical field [0001] The invention belongs to the technical field of insect genetics, and in particular relates to a method for constructing adversity-resistant near-isogenic lines of parthenogenetic insects. Background technique [0002] In insect resistance research, sensitive strains and resistant strains of insects are usually used as test insect materials to study and compare the differences in physiology, biochemistry and biology between the two groups of insects, so as to clarify the development of insecticide resistance in insects. Changes in traits and insecticide targets provide the basis for resistance management. [0003] In the prior art, insects of resistant strains and insects of sensitive strains are reared in isolation, insects of resistant strains are mostly obtained by selecting insecticides for sensitive strains, and obtained after successive generations, insects of sensitive strains are not exposed to any pesticides, The two populations obtained by th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/0335
Inventor 吴青君苑广迪万岩然何秉青李晓宇
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI