Composite microbial agent for treating contaminated water and preparation method thereof
A technology of composite microbial agent and water body, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of lack of self-purifying microorganisms, lack of self-purifying function, and small amount of bacteria, etc., to achieve short cultivation period, bacteria The effect of good species diversity and strong stress resistance
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[0042] The present invention also provides a kind of preparation method of the composite microbial bacterial agent of treating polluted water body, comprises the following steps:
[0043] S10: configuring culture medium;
[0044] S20: Inoculation: add Bacillus subtilis, Paracoccus denitrificans, Clostridium papiersolvee, Bacillus licheniformis, photosynthetic bacteria, actinomycetes, denitrifying bacteria, nitrobacterium and nitrosobacillus to the culture medium prepared in S10 to ferment together Afterwards, filter to obtain filtrate;
[0045] S30: Dilute the filtrate prepared in S20 with a protective solution.
[0046]Preferably, in step S10, the culture medium is prepared in parts by weight, including 15-18 parts of sweet potato powder, 20-30 parts of tempeh, 3-5 parts of beef extract, 3.5-4.5 parts of yeast extract, and 0.5-1 part of sodium alginate , 0.01-0.03 parts of dipotassium hydrogen phosphate, 0.02-0.05 parts of glutamic acid, 0.5-1 part of sodium chloride, 0.01-...
Embodiment 1
[0059] The percentage by weight of microorganisms in the compound microbial bacterial agent for controlling polluted water is as follows: a mixture of 3% Rhodopseudomonas zazena, 35% Bacillus subtilis and Bacillus licheniformis, wherein the colony ratio of Bacillus subtilis and Bacillus licheniformis is 1 : 1. Biological enzyme composed of 25% Nitrosobacillus, 25% Nitrobacter, 5% Denitrifying Bacillus and 7% lipase and subtilisin.
Embodiment 2
[0061] The percentage by weight of microorganisms in the compound microbial bacterial agent for controlling polluted water is as follows: the mixture of 15% Rhodopseudomonas zenezensee, 20% Bacillus subtilis and Bacillus licheniformis, wherein the colony ratio of Bacillus subtilis and Bacillus licheniformis is 2 : 1. Biological enzyme composed of 20% Nitrosobacillus, 15% Nitrobacter, 25% Denitrifying Bacillus and 5% lipase and subtilisin.
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