A kind of preparation method of natural p-aminobenzoic acid and derivatives thereof
A technology for para-aminobenzoic acid and derivatives, which is applied in the field of biomedicine, can solve problems such as environmental pollution, and achieve the effects of being environmentally friendly, simple and safe in preparation method, and easy for industrial production.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0014] The preparation method of natural p-aminobenzoic acid and derivative thereof provided by the invention, its steps are:
[0015] 1) Liquid culture and fermentation product processing: the fungus Termitomyces albuminosus The slant strains were first inoculated in a Erlenmeyer flask containing 150mL potato liquid medium for activation. The activation conditions were 230 r / min, 28°C, and cultured for 7 days, followed by large-scale liquid fermentation. The medium formula was: : Each liter of water contains 200 g of potatoes, 20 g of glucose, natural pH, 0.1 Mpa, sterilized at 121 ℃ for 30 minutes, and cultured in a constant temperature shaker at 25-28 ℃ and 180-230 r / min. After 15 days of fermentation, the mycelium was separated from the fermentation broth by centrifugation. The fermented liquid was extracted with an equal volume of ethyl acetate, the obtained extract was dehydrated with anhydrous sodium sulfate, and then concentrated in vacuo with a rotary evaporator at 4...
Embodiment 1
[0019] Use potato glucose medium (containing 200g of potato per liter of water, 20g of glucose, natural pH, 0.1Mpa, sterilized at 121°C for 30min), and the activated strain Termitomyces albuminosus 37 L of liquid fermentation was cultured on a shaker at 28°C for 15 days. After the bacterial cells were filtered, the fermentation broth was concentrated to 1 L, and the fermentation broth was extracted with ethyl acetate. The ethyl acetate part was dehydrated with anhydrous sodium sulfate and then concentrated to obtain an organic crude extract (3.1 g).
[0020] The 3.1 g organic crude extract obtained in the previous step was fully dissolved in methanol, and subjected to reverse-phase silica gel (170 g) column chromatography, and eluted with 30% methanol water for 2 L at a flow rate of 15 mL / min. Collect 280 mL, concentrate in vacuo with a rotary evaporator, take samples separately for thin-layer chromatography analysis and combine (developing solvent is chloroform:methanol=10:1,...
Embodiment 2
[0027] Use potato glucose medium (containing 200g of potato per liter of water, 20g of glucose, natural pH, 0.1Mpa, sterilized at 121°C for 30min), and the activated strain Termitomyces albuminosus 37 L of liquid fermentation was cultured on a shaker at 28°C for 15 days. After the bacterial cells were filtered, the fermentation broth was concentrated to 1 L, and the fermentation broth was extracted with ethyl acetate. The ethyl acetate part was dehydrated with anhydrous sodium sulfate and then concentrated to obtain an organic crude extract (3.1 g).
[0028] Dissolve 3.1 g of the crude organic extract obtained in the previous step in methanol, perform reverse phase silica gel (170 g) column chromatography, and elute 2 L with 30% methanol water at a flow rate of 15 mL / min. Collect 280 mL, concentrate in vacuo with a rotary evaporator, take samples in separate tubes for thin-layer chromatography detection and combine (developing solvent is chloroform:methanol=10:1, chromogenic r...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More