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Metabonomics analytical method for tobacco pollen based on chromatography-mass spectrometry

A technology of gas chromatography mass spectrometry and metabolomics, which is applied to the analysis of materials, scientific instruments, material separation, etc.

Inactive Publication Date: 2016-10-12
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, people have carried out a lot of research on tobacco quality, disease resistance, insect resistance, etc. These studies are mainly carried out from the aspects of genes and environment, but there are few related reports on the research from the perspective of metabolomics

Method used

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  • Metabonomics analytical method for tobacco pollen based on chromatography-mass spectrometry
  • Metabonomics analytical method for tobacco pollen based on chromatography-mass spectrometry
  • Metabonomics analytical method for tobacco pollen based on chromatography-mass spectrometry

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Embodiment 1

[0071] ——Study on metabolomics of tobacco pollen in different varieties (Yunyan 87 and Yunyan 97)

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Abstract

The invention discloses a metabonomics analytical method for tobacco pollen based on chromatography-mass spectrometry. The metabonomics analytical method comprises the following steps: sample pretreatment, chromatography-mass spectrometry separation, structural identification of metabolites, quantitative analysis of the metabolites, and data analysis. According to the metabonomics analytical method, polar and nonpolar metabolites of the tobacco pollen are respectively extracted by virtue of a polar solvent and a nonpolar solvent, and all the metabolites collected by virtue of the chromatography-mass spectrometry are identified by virtue of a retention index and a mass spectrometry database and are quantitatively analyzed by virtue of a non-target quantitative analysis method. By virtue of the establishment of the method, important references are provided for the implementation of relevant research on the metabonomics of the tobacco pollen.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, and in particular relates to a tobacco pollen metabolomics analysis method based on gas chromatography mass spectrometry. Background technique [0002] Metabolomics is a qualitative and quantitative study of the dynamic multi-parameter metabolic responses of biological systems to pathophysiological stimuli and changes in genes and environments. Metabolomics is a research field that has developed rapidly in recent years. Since the concept of metabolomics was proposed in 1997, it has achieved fruitful results in the fields of plant, animal and microbial research, especially in the research of plant metabolomics. Universities and research institutes have carried out related research work. [0003] The analytical methods used in metabolomics research mainly include nuclear magnetic resonance spectroscopy, liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry, and...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/025
Inventor 李勇逄涛师君丽杨大海
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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