A Rapid Protein Detection System Based on Centrifugal Platform

Abstract

The invention discloses a rapid protein detection system based on a centrifugal platform and aims to achieve the purposes that automatic control over micro channels is achieved through the centrifugal platform, the operation procedure is integrated to the same micro-fluidic chip, and thus automated, rapid and low-cost detection of protein is achieved. According to the technical scheme, the rapid protein detection system comprises a rotating shaft fixedly connected with a motor, the rotating shaft is coaxially provided with the micro-fluidic chip and a sliding disc from top to bottom in sequence, the micro-fluidic chip comprises silica gel PDMS, the upper surface of the silica gel PDMS is provided with a plurality of cavities, the multiple cavities are communicated through the micro channels, the upper surface of the silica gel PDMS is covered with an organic glass plate PMMA, the silica gel PDMS and the organic glass plate PMMA are fixedly connected with the rotating shaft, the sliding disc is arranged on the rotating shaft in a sleeved mode, the upper end of the sliding disc is fixedly provided with a plurality of elastic pins for switching off or switching on the micro channels, the lower end of the sliding disc is fixedly provided with a centrifugal mechanism, the centrifugal mechanism is fixedly connected with the rotating shaft, and an elastic component is arranged between the centrifugal mechanism and the sliding disc.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips, and relates to a protein rapid detection system based on a centrifugal platform. Background technique [0002] Microfluidic chip integrates many processes and steps of biochemical analysis on a single chip. It has the remarkable characteristics of fast detection speed, low reagent consumption and high throughput. Therefore, it has been researched and concerned by scholars from all over the world in recent years Compared with detection methods, microfluidic chips have irreplaceable advantages. [0003] At present, in the microfluidic chip-based detection system, the power source powered by the liquid flow is difficult to be stably integrated on the protein chip, and the conventional implementation method is likely to cause contamination to the reagents in the chip, thereby affecting the accuracy of the detection results. Moreover, many biochemical operations need to be integrated on a ...

AI Technical Summary

Problems solved by technology

The most commonly used method at present is to use polystyrene to form a link with the antibody to immobilize the antibody. This method requires a long reaction time and many steps, which is contrary to the required fast and efficient detection.

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