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A method of measuring the content of astragaloside in heart-tonifying pulse-restoring granules

A determination method, the technology of astragaloside IV, is applied in the field of determination of astragaloside IV content in Yixin Fumai Granules, which can solve the problems that cannot fully reflect the quality of the product

Inactive Publication Date: 2016-10-19
TIANJIN TASLY PHARMA CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Due to the large number of active ingredients, sensitive detection methods are needed to detect the active ingredients of Yixin Fumai Granules, but the existing detection methods only include traits, 2 color identifications and related detection items under inspection items, and no medicinal materials are tested. Qualitative or quantitative detection can not fully reflect the quality status of the product. The present invention has established a method for the determination of astragaloside IV in the product for the monarch drug Astragalus, which fills the gap in the quantitative control of Yixin Fumai Granules, so that the product can be more accurately controlled. Effective quality analysis can more comprehensively reflect the quality status of the product and ensure the quality stability of the product. At the same time, this method saves testing costs

Method used

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  • A method of measuring the content of astragaloside in heart-tonifying pulse-restoring granules
  • A method of measuring the content of astragaloside in heart-tonifying pulse-restoring granules
  • A method of measuring the content of astragaloside in heart-tonifying pulse-restoring granules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0143] Example 1 Take Yixin Fumai Granules numbered as Test 3, and measure the content of astragaloside IV

[0144] Step 1, preparation of reference substance solution

[0145] Take an appropriate amount of astragaloside IV reference substance, weigh it accurately, add 70% methanol to make it contains 0.20 mg of astragaloside IV per 1 ml.

[0146] Step 2, the preparation of the test solution, the method is as follows: take about 3.0 g of Yixin Fumai Granules, accurately weighed, put it into a 50ml Erlenmeyer flask, add 15ml of 4% ammonia water precisely, and ultrasonically treat it for 25 minutes to fully dissolve it. Centrifuge (3000 rpm, 10min), pipette 10ml of the supernatant precisely, and pass through the treated C18 solid phase extraction column (500mg, first eluted with 5ml of methanol, then eluted with 5ml of water) at a speed of 1ml / min , eluted with 5ml of water, discarded the eluent, then slowly eluted with 2ml of methanol into a 2ml volumetric flask, diluted with ...

Embodiment 2

[0150] Example 2 Take the Yixin Fumai Granules numbered as Test 1, and measure the content of Astragaloside IV

[0151] Step 1, preparation of reference substance solution

[0152] Take an appropriate amount of astragaloside IV reference substance, weigh it accurately, add 70% methanol to make it contains 0.16-0.24mg of astragaloside IV per 1ml.

[0153] Step 2, the preparation of the test solution, the method is as follows: take about 3.0 g of Yixin Fumai Granules, accurately weighed, put it into a 50ml Erlenmeyer flask, add 15ml of 4% ammonia water precisely, and ultrasonically treat it for 25 minutes to fully dissolve it. Centrifuge (3000 rpm, 10min), pipette 10ml of the supernatant precisely, and pass through the treated C18 solid phase extraction column (500mg, first eluted with 5ml of methanol, then eluted with 5ml of water) at a speed of 1ml / min , eluted with 5ml of water, discarded the eluent, then slowly eluted with 2ml of methanol into a 2ml volumetric flask, dilute...

Embodiment 3

[0157] Example 3 Take the Yixin Fumai Granules numbered as Test 2, and measure the content of Astragaloside IV

[0158] Step 1, preparation of reference substance solution

[0159] Take an appropriate amount of astragaloside IV reference substance, weigh it accurately, add 70% methanol to make it contains 0.16-0.24mg of astragaloside IV per 1ml.

[0160] Step 2, the preparation of the test solution, the method is as follows: take about 3.0 g of Yixin Fumai Granules, accurately weighed, put it into a 50ml Erlenmeyer flask, add 15ml of 4% ammonia water precisely, and ultrasonically treat it for 25 minutes to fully dissolve it. Centrifuge (3000 rpm, 10min), pipette 10ml of the supernatant precisely, and pass through the treated C18 solid phase extraction column (500mg, first eluted with 5ml of methanol, then eluted with 5ml of water) at a speed of 1ml / min , eluted with 5ml of water, discarded the eluent, then slowly eluted with 2ml of methanol into a 2ml volumetric flask, dilute...

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Abstract

The invention relates to a method of measuring an effective component in a traditional Chinese medicine, and particularly relates to a method of measuring the content of astragaloside in heart-tonifying pulse-restoring granules. The method includes a step of preparing a reference substance solution, namely a step of measuring a proper amount of an astragaloside reference substance and adding water-containing methanol or a mobile phase to prepare into a solution containing 0.16-0.24 mg1 / mL of astragaloside; a step of preparing a sample solution to be measured, namely a step of weighting the heart-tonifying pulse-restoring granules, extracting with an alkali water solution having a concentration of 2-5%, adding the extract liquid into a solid phase extraction column, and eluting with methanol, and a step of measuring, namely a step of separately injecting 5-20 muL of the reference substance solution and the sample solution to be measured into a high performance liquid chromatograph.

Description

technical field [0001] The invention relates to a method for determining the content of active ingredients in traditional Chinese medicine preparations, in particular to a method for determining the content of astragaloside IV in Yixin Fumai granules. Background technique [0002] Yixin Fumai Granule is a drug produced by Tianjin Tasly (Liaoning) Pharmaceutical Co., Ltd. Its main functions are to nourish qi and nourish yin, activate blood and restore pulse; it is used for deficiency of both qi and yin, internal resistance of heart and blood, chest pain, chest tightness and discomfort , palpitations and pulse knots. It has the characteristics of high drug loading, fast onset, no peculiar smell, and easy to carry. Yixin Fumai Granules is a compound preparation composed of 6 medicinal materials: Sun-dried Ginseng, Astragalus, Radix Ophiopogon japonicus, Schisandra, Salvia Miltiorrhiza, and Chuanxiong. [0003] Raw sun-dried ginseng: It has the functions of nourishing vitality...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 侯春莲刘海涛高展葛丹丹孙玉侠曹凤兰徐立元赵光燃杨建会杨雪梅
Owner TIANJIN TASLY PHARMA CO LTD
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