Preparation method of feed additive nano small peptide chelated copper
A feed additive and small peptide chelation technology, which is applied in the direction of animal feed, protein composition of vegetable seeds, additional food elements, etc., can solve the problem of high price of small peptide chelates, and achieve improved immunity, high purity, The effect of low production cost
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[0021] A preparation method for feed additive nano small peptide chelated copper, comprising the steps of:
[0022] (1) Preparation of buffer solution: Prepare 0.2M disodium hydrogen phosphate solution and 0.1M citric acid solution for later use;
[0023] (2) Preparation of dry peptide powder: Take 1.6 kg of soybean meal powder (particle size ≤0.6 mm) and dissolve it in 10 L, pH=4.3, 0.2M disodium hydrogen phosphate solution and 0.1M citric acid solution in a buffer solution, at 40°C Stirring at low temperature for 12 min; heat treatment at 90°C for 17.5 min; then adding alkaline protease and flavor protease [Alcalase alkaline protease (liquid) 2.4 L, Flavorzyme flavor protease 500 MG, both provided by Novozymes Investment Co., Ltd., two The mass ratio of the two enzymes is 1:1], at 55°C, pH 7.0 (adjust the pH with 0.2M disodium hydrogen phosphate solution), and the enzyme concentration is 10.5% (that is, the two enzymes account for 10.5% of the mass of soybean meal powder). ...
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