Digital nucleic acid amplification instrument

Abstract

The invention provides a digital nucleic acid amplification instrument. The digital nucleic acid amplification instrument comprises a case; a temperature controller and a fan module are arranged in the case; the fan module comprises a thermoelectric cooler; the thermoelectric cooler is arranged at one side of the case; the temperature controller is arrange at the other side of the case at a position close to the thermoelectric cooler, electrically connected with the thermoelectric cooler and used for controlling the temperature of the temperature controller in real time; and the thermoelectric cooler is arranged in the case in virtue of a push-and-pull structure which can enter the case from the front and exit from the back or enter the case from the back and exit from the front. The digital nucleic acid amplification instrument is simple in structure and convenient to operate; the arrangement manner of each part is optimal, so the space of the integral instrument is reduced and production cost is lowered; and the thermoelectric cooler and the temperature controller can realize temperature circulation, accurately control the denaturation, annealing and extension temperature, time and cycle numbers of a template and realize accurate regulation and control in rapid rise and reduction of temperature in amplification.

Description

technical field [0001] The invention relates to the technical field of nucleic acid amplification, in particular to a digital nucleic acid amplification instrument. Background technique [0002] Nucleic acid amplification technology, especially digital polymerase chain reaction technology (Polymerase Chain Reaction referred to as PCR), is a molecular biological technology used to amplify specific DNA molecular fragments. It uses DNA molecules as templates and consists of a pair of artificially synthesized Specific oligonucleotide primers can rapidly amplify specific DNA molecular fragments through the enzymatic reaction of DNA polymerase, and play an extremely important role in biology. As its applications become more and more extensive, the requirements for nucleic acid amplification instruments (such as PCR instruments) are getting higher and higher. For example, for PCR machines, compared with traditional PCR technology, digital PCR technology is more and more widely use...

AI Technical Summary

Problems solved by technology

But above-mentioned invention is not high to the precision of reagent temperature regulation, and can't realize the regulation and control of rapidly raising and lowering temperature

Moreover, the overall structure of the PCR instrument is not compact enough, which affects heat dissipation and is not conducive to installation and maintenance.

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