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Sargassum leizhouense seedling large-scale suspension over-summer cultivation method based on generative propagation

A technology of sexual reproduction and cultivation method, which is applied in the field of large-scale suspension summer cultivation of Sargassum seedlings in Leizhou, to achieve the effects of saving seedling breeding sites, saving costs and avoiding damage

Inactive Publication Date: 2016-11-09
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the above technical problems, the present invention provides a large-scale suspension summer cultivation method for Sargassum leizhou seedlings based on sexual reproduction, to solve the summer cultivation management in the process of large-scale cultivation of Sargassum seedlings, reduce costs and seedling risks, Improving the success rate of seedling breeding and other issues

Method used

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  • Sargassum leizhouense seedling large-scale suspension over-summer cultivation method based on generative propagation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The large-scale suspended summer culture method of Leizhou Sargassum seedlings based on sexual reproduction includes the following steps: Step 1. Under natural conditions, the temperature of the cultured water body is 28°C, and the surface light intensity of the cultivated water body is shaded by a single-layer sunshade net 500μmol·m -2 the s -1 At the same time, clean, mature but not ejaculated and ovulated Sargassum leizhou algae were evenly mixed at a mass ratio of male to female of 4:1, dispersed in the culture water with a salinity of 29, and cultivated with strong aeration, which means that every hundred Inflate 28mL per second in the water body, the water body is in a state of motion, and the appearance of the entire water body is tumbling; step 2, the fertilized eggs and free seedlings that fell off the wall and bottom of the culture container in step 1 are brushed off with a soft brush and collected , followed by 40 mesh, 60 mesh and 300 mesh sieve tulle three...

Embodiment 2

[0026] The large-scale suspended summer culture method for Sargassum sargassum seedlings in Leizhou based on sexual reproduction comprises the following steps: Step 1. Under natural conditions, the temperature of the cultured water body is 30°C, and the light intensity on the surface of the cultivated water body is shaded by a single-layer sunshade net 550μmol·m -2 the s -1 At the same time, clean, mature but not ejaculated and ovulated Sargassum leizhou algae were evenly mixed at a mass ratio of male to female of 4:1, dispersed in the culture water body with a salinity of 32, and cultivated with strong aeration. Inflate 28mL per second in the water body, the water body is in a state of motion, and the appearance of the entire water body is tumbling; step 2, the fertilized eggs and free seedlings that fell off the wall and bottom of the culture container in step 1 are brushed off with a soft brush and collected , followed by 40 mesh, 60 mesh and 300 mesh sieve tulle three-sta...

Embodiment 3

[0028] The seedling culture that obtains under embodiment 2 and traditional attached culture condition contrasts, and the result is as shown in the table below:

[0029]

[0030]The results in the above table show that after the fertilized eggs are discharged, the attached seedlings grow slightly faster than the suspended seedlings in the early stage of outdoor cultivation (within 28 days), but as the cultivation time prolongs, the suspended seedlings grow faster than the attached seedlings. In the later stage of indoor temperature-controlled cultivation, the suspended seedlings are inflated and cultivated in the container, while the attached seedlings are bound to the seedling curtain for cultivation. Compared with the attached seedlings, the suspended seedlings are more uniform in light due to the up and down rolling, so the growth index of the suspended seedlings should be higher than that of the attached seedlings. It is better than the growth index of attached seedlings...

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Abstract

The invention discloses a Sargassum leizhouense seedling large-scale suspension over-summer cultivation method based on generative propagation. The method includes the steps that male and female Sargassum leizhouense species are mixed evenly, the mixture is dispersed into cultivation water in a cultivation vessel, and high-aeration cultivation is carried out till zygotes fall off naturally; then, the zygotes and free seedlings are collected and filtered, and pure zygotes and free seedlings are obtained; then, the pure zygotes and free seedlings are transferred into the cultivation water, and slight-aeration cultivation is carried out till all the zygotes and free seedlings grow into primary seedlings; then, high-aeration cultivation is carried out on the primary seedlings in the cultivation water till the primary seedlings grow into seedlings of a cultivation specification; finally, high-aeration cultivation continues to be carried out on the seedlings of a cultivation specification in the cultivation water, and preservation is carried out. Compared with the prior art, by means of the method, the production intensity of weaving and treating an adherence substrate is relieved, the cost is reduced, damage caused by improper seedling growing device treatment to seedlings is avoided, the loss of seedlings is reduced, management is easy in the seedling growing process, adherence of unwanted algae is reduced, the seedling growing site is saved, the site construction cost is reduced, and the cultivation intermediate links are simplified.

Description

technical field [0001] The invention relates to the technical field of Sargassum seedling cultivation, in particular to a large-scale suspension summer cultivation method for Leizhou Sargassum seedlings based on sexual reproduction. Background technique [0002] Leizhou Sargassum has wide leaves and has broad application prospects in the restoration of marine ecosystems and the construction of marine pastures. It is abundant in the coastal areas of Guangdong and Guangxi. However, in recent years, the natural resources of this algae have been obviously negatively affected by natural or human factors, and the amount of resources is decreasing year by year, so it is imminent to carry out artificial breeding. [0003] In domestic research on Sargassum seaweed cultivation, in addition to using its rhizomes and tissue blocks for vegetative seedling cultivation, sexual reproduction seedlings are mainly used. However, in the process of large-scale seedling cultivation based on sexua...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G33/00
CPCA01G33/00Y02A40/80
Inventor 赵素芬孙会强
Owner GUANGDONG OCEAN UNIVERSITY
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