A detection method and device for biomolecular interaction

A biomolecule and detection method technology, applied in the field of biomolecular interaction detection, can solve problems such as unsatisfactory improvement in sensitivity, and achieve the effects of strong interference suppression ability, improved sensitivity, and simple device

Active Publication Date: 2019-03-01
广州广华深启科技有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the increase in sensitivity is still not ideal

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  • A detection method and device for biomolecular interaction
  • A detection method and device for biomolecular interaction
  • A detection method and device for biomolecular interaction

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Embodiment Construction

[0012] The present invention will be described in further detail below in combination with specific embodiments and with reference to the accompanying drawings.

[0013] The biomolecular interaction detection method based on the weak measurement proposed by the present invention is a brand-new detection method which applies the weak measurement method to the biomolecular interaction detection. Using the principle of weak measurement, the phase signal will be amplified, which has high sensitivity to surface information, and this weak measurement sensor has extremely high sensitivity.

[0014] Such as figure 1 Shown is a schematic diagram of the principle of the detection device for biomolecular interactions. The detection device includes a light source, a first polarizer, a biosensing unit, a second polarizer, a spectrometer and a processing unit. The light emitted by the light source first enters the front selection optical path of the weak measurement system, and then enter...

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Abstract

The invention discloses a bio-molecule interaction detection method and a bio-molecule interaction detection apparatus. The detection method comprises the following steps: 1, a light source with the spectrum width being 30 nm or above and the light intensity being 1 mW or above is selected as a light source; 2, lights emitted by the light source go through a polaroid, irradiate a biological sensing unit, are totally reflected in the biological sensing unit, go through a second polaroid, and then are emitted, wherein an included angle between the polarization direction of the first polaroid and the polarization direction of the second polaroid is pi / 4 + / -0.05 radian; 3, a spectrometer records the spectral central wavelength change of the emitted lights going through a buffer solution and molecules to be detected on the biological sensing unit; and 4, calculating the concentration of the passed molecules to be detected on the biological sensing unit according to the spectral central wavelength change. The bio-molecule interaction detection method and bio-molecule interaction detection apparatus have high detection sensitivity.

Description

【Technical field】 [0001] The invention relates to the technical field of molecular detection, in particular to a detection method and device for biomolecular interaction. 【Background technique】 [0002] The detection of biomolecular interactions has become a research hotspot in the field of life science and technology. At present, the technologies for detecting biomolecular interactions use parameters such as light intensity and polarization, and some use light phase changes for detection. Since the phase of light itself is more sensitive than light intensity and polarization, the detection based on the phase of light is more sensitive than the detection based on light intensity and polarization. However, even though the optical phase is more sensitive, its sensitivity to the change of the refractive index of the biosensing unit still needs to be continuously improved. Among the existing techniques for detecting molecular interactions using optical phase, there are schemes...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/41
CPCG01N21/41G01N2201/0683
Inventor 何永红张怡龙李东梅
Owner 广州广华深启科技有限责任公司
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