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A kind of in vitro rapid propagation method of gerbera

A gerbera, in vitro technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of difficulty in cultivating the first generation of gerbera gerbera, achieve rapid in vitro propagation, and increase the survival rate Effect

Active Publication Date: 2018-05-11
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the previous experiments, the survival rate of the conventional primary culture was less than 10%, which fully proved that the primary culture of G.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: a kind of method of gerbera gerbera in vitro rapid propagation method, concrete steps are as follows:

[0020] (1) Defiberization treatment of explants: select potted domesticated gerbera gerbera with excellent performance in the greenhouse, pour the prepared hormone into the culture pot, and then place it in the greenhouse for 1 month. The concentration of watering hormone in fiber treatment was 0.5mg / L6-BA+0.1 mg / LNAA.

[0021] (2) Primary culture: use the shortened stems with vigorous growth and hairless new leaves obtained in step (1) as explants, cut to a length of 1.5 cm, rinse with tap water for 1 hour, dry and set aside for later use. Soak in 75% alcohol for 60s in a clean workbench, soak in 0.1% tribute for 10min, then wash 5 times with sterile distilled water, cut off both ends of the tissue with a scalpel, leave a length of 1.0-1.5cm and inoculate it into the primary culture medium , 14 hours of light every day, the light intensity is 1000lux, ...

Embodiment 2

[0025] Embodiment 2: a kind of method of gerbera gerbera in vitro rapid propagation method, concrete steps are as follows:

[0026] (1) Defiberization treatment of explants: select potted domesticated gerbera gerbera with excellent performance in the greenhouse, pour the prepared hormone into the culture pot, and then place it in the greenhouse for 1 month. The concentration of watering hormone in fiber treatment was 1mg / L6-BA+0.5 mg / LNAA.

[0027] (2) Primary culture: use the shortened stems obtained in step (1) that grow vigorously and have hairless new leaves as explants, cut them to a length of 2 cm, rinse them with water for 1.5 hours, dry them for later use, and store them in ultra-clean Soak in 75% alcohol for 40s in the workbench, soak in 0.1% tribute for 5min, wash with sterile distilled water for 3 times, cut off both ends of the tissue with a scalpel, leave a length of 1.0-1.5cm, and then inoculate into the primary culture medium , placed under the conditions of 12...

Embodiment 3

[0031] Embodiment 3: a kind of method of gerbera gerbera in vitro rapid propagation method, concrete steps are as follows:

[0032] (1) Defiberization treatment of explants: select potted domesticated gerbera gerbera with excellent performance in the greenhouse, pour the prepared hormone into the culture pot, and then place it in the greenhouse for 1 month. The concentration of watering hormone in fiber treatment was 0.1 mg / L6-BA+0.5 mg / LNAA.

[0033] (2) Primary culture: use the shortened stems obtained in step (1) that grow vigorously and have hairless new leaves as explants, cut them to a length of 2 cm, rinse them with water for 1.5 hours, dry them for later use, and store them in ultra-clean Soak in 75% alcohol for 45s in the workbench, soak in 0.1% tribute for 6min, wash with sterile distilled water for 3 times, and then put it under the condition of 12 hours of light every day, the light intensity is 1500lx, and the culture temperature is 25℃. Until the formation of cl...

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Abstract

The invention discloses an in-vitro rapid propagation method for Gerbera delavayi Franch. Large number of high quality seedlings of the Gerbera delavayi Franch can be rapidly obtained by virtue of a tissue culture technology, and the method comprises the following concrete steps of: (1) carrying out defiber treatment on an explant; (2) carrying out primary culture; (3) carrying out induction of cluster buds; (4) carrying out rooting culture; and (5) hardening seedlings and transplanting. The in-vitro rapid propagation method disclosed by the invention has the advantages that an in-vitro rapid propagation system of the Gerbera delavayi Franch is established, artificial planting of the Gerbera delavayi Franch can be promoted, survival rate of the primary culture of the Gerbera delavayi Franch is improved, and in-vitro rapid propagation of the Gerbera delavayi Franch is realized.

Description

technical field [0001] The invention relates to a method for in vitro rapid propagation in plant biotechnology, in particular to a method for in vitro rapid propagation of the fibrous plant gerbera gerbera. Background technique [0002] Hook bud gerbera ( Gerbera delavayi Franch) is a perennial herb of the genus Gerbera in the Compositae family. It is distributed in Yunnan Province, southern Sichuan Province and northern Vietnam. It is called "fire grass" because its leaves were used by the ancients to ignite fire. The application value of gerbera gerbera is mainly manifested in: (1) Textile, the felt fiber on the back of gerbera gerbera leaves is the raw material for weaving fireweed clothing by ethnic minority people in Southwest my country. Huocao clothes are warm in winter and cool in summer, and become whiter with washing. They are the symbol of love and wisdom of the Yi, Lisu, Bai, Naxi, Zhuang and other ethnic groups in southwest my country, and are often used as a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 郑伟王谈笑徐晓丹陈菁王炜
Owner KUNMING UNIV OF SCI & TECH