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A fermentation culture method capable of increasing the yield of subtilis lipopeptide sodium

A technology of sodium subtilisin lipopeptide and fermentation culture is applied in the field of fermentation and culture that can increase the yield of sodium subtilisin lipopeptide, and achieves the effects of promoting the synthesis of lipopeptide subtilisin, increasing the yield, and satisfying industrialized large-scale production.

Active Publication Date: 2019-02-26
PROYA COSMETICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing research, C in the subtilisin obtained by fermentation 15 The content of components is only 50~60%

Method used

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  • A fermentation culture method capable of increasing the yield of subtilis lipopeptide sodium
  • A fermentation culture method capable of increasing the yield of subtilis lipopeptide sodium
  • A fermentation culture method capable of increasing the yield of subtilis lipopeptide sodium

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Experimental program
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Effect test

Embodiment 1

[0028] A fermentation culture method capable of increasing the output of subtilis lipopeptide sodium, the following steps are adopted:

[0029] (1) Seed bacteria culture: take Bacillus subtilis BS-37 as the starting strain, transfer the preserved strains to LB plates, and place them in a 37°C incubator to activate for 24 hours; The loops were inoculated in the seed liquid medium, cultured on a shaking table at 28 °C and 210 rpm / min for 24 h, and then used as seed bacteria for future use. The composition of seed liquid medium is: beef extract 4.0 g / L, peptone 12.0 g / L, yeast extract 6.0 g / L, NaCl 3.0 g / L, glucose 8.0 g / L, pH 6.8.

[0030] (2) Fermentation culture: Inoculate the seed bacteria cultivated in step (1) with 24.0 g of glucose, 2.4 g of seaweed oil, 0.8 g of rice bran wax, and 6.0 g of D-glutamic acid at an inoculation volume of 3.0% by volume. g, D-leucine 1.0 g basic inorganic salt medium (MMSM medium) (see Table 4 for the formula), cultured on a shaker at 34°C and...

Embodiment 2

[0035] A fermentation culture method capable of increasing the output of subtilis lipopeptide sodium, the following steps are adopted:

[0036] (1) Seed bacteria culture: take Bacillus subtilis BS-37 as the starting strain, transfer the preserved strains to LB plates, and place them in a 37°C incubator to activate for 24 hours; The loop was inoculated in the seed liquid medium, cultured on a shaker at 28°C and 210 rpm / min for 24 h, and then used as a seed fungus for later use. The composition of seed liquid medium is: beef extract 4.0 g / L, peptone 12.0 g / L, yeast extract 6.0 g / L, NaCl 3.0 g / L, glucose 8.0 g / L, pH 6.6.

[0037] (2) Fermentation culture: Inoculate the seed bacteria cultivated in step (1) according to the inoculum size of 5.5% by volume, and insert them into the fermentation medium supplemented with 36.0 g of glucose, 3.8 g of seaweed oil, 2.4 g of rice bran wax, and 4.0 g of D-glutamic acid. g, D-leucine 0.5 g basal inorganic salt medium (MMSM medium) (see Tabl...

Embodiment 3

[0042] A fermentation culture method capable of increasing the output of subtilis lipopeptide sodium, the following steps are adopted:

[0043] (1) Seed bacteria culture: take Bacillus subtilis BS-37 as the starting strain, transfer the preserved strains to LB plates, and place them in a 37°C incubator to activate for 24 hours; The loop was inoculated in the seed liquid medium, cultured on a shaker at 28°C and 210 rpm / min for 24 h, and then used as a seed fungus for later use. The composition of seed liquid medium is: beef extract 4.0 g / L, peptone 12.0 g / L, yeast extract 6.0 g / L, NaCl 3.0 g / L, glucose 8.0 g / L, pH 7.0.

[0044] (2) Fermentation culture: Inoculate the seed bacteria cultivated in step (1) with 50.0 g of glucose, 15.0 g of seaweed oil, 5.0 g of rice bran wax, and 5.0 g of D-glutamic acid at an inoculum size of 8.0% by volume. g, D-leucine 1.0 g basal inorganic salt medium (MMSM medium) (see Table 6 for the formula), cultured on a shaker at 36°C and 280 rpm / min fo...

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Abstract

The invention relates to a fermentation culture method capable of increasing the yield of bacillus subtilis lipopeptide sodium. The fermentation culture method is characterized in that 1, seed bacterium culture is conducted by taking Bacillus subtilis as an original strain; 2, fermentation culture is conducted, wherein the well cultured seed bacteria in the first step are inoculated into a fermentation medium according to the inoculation quantity of 2.5%-12% by volume, the fermentation medium takes glucose, algal oil and rice bran wax as carbon sources, and the total mass concentration of D-glutamic acid and D-leucine ranges from 3 g / L to 8 g / L; 3, alkalization is conducted to obtain a salt, and a crude bacillus subtilis lipopeptide sodium product is obtained. The prepared bacillus subtilis lipopeptide sodium is high in yield, and the surface activity of the bacillus subtilis lipopeptide sodium is higher.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a fermentation culture method capable of increasing the yield of lipopeptide sodium subtilis. technical background [0002] Biosurfactants are a class of biomacromolecular substances with surface activity produced by microorganisms, which are mainly produced by microbial fermentation and enzyme catalysis. Compared with traditional chemically synthesized surfactants, biosurfactants have stronger interfacial activity, and also have the advantages of being non-toxic, biodegradable, and non-polluting. [0003] According to differences in chemical composition and microbial origin, biosurfactants can be divided into five categories: glycolipids, fatty acids and phospholipids, lipopeptides and lipoproteins, polymeric biosurfactants and special biosurfactants. Among them, surfactin is a lipopeptide biosurfactant produced by Bacillus subtilis, which is mostly u...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/04C12R1/125
CPCC12P21/02
Inventor 黄洁芳毕永贤王丽娜陈斌陈玉燕张金龙蒋丽刚金汉坤
Owner PROYA COSMETICS
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