Preparation method of liquid-based cell

Abstract

The invention discloses a preparation method of a liquid-based cell. The preparation method comprises the following steps: step 1, taking a cell smearing device, wherein the cell smearing device comprises a tray, a preparation warehouse fixed on the tray, a glass slide positioned on the lower end surface of the preparation warehouse and used for plugging the lower end of the preparation warehouse, a filter membrane detachably arranged in the preparation warehouse and arranged in the circumferential direction of the preparation warehouse, a filter chamber formed in the preparation warehouse and positioned above the filter membrane and a cleaning chamber formed in the preparation warehouse and positioned under the filter membrane; adding cleaning liquid into the preparation warehouse and enabling all the cleaning liquid to enter the cleaning chamber and the liquid level of the cleaning liquid to be flush with the filer membrane; step 2, taking and adding a mixture of a sample and a preservation solution into the filter chamber, enabling the mixture to pass through the filter membrane to enter the cleaning chamber and enabling a cell to settle to the bottom of the cleaning chamber; step 3, removing the filter membrane as well as impurities on the filter membrane and enabling natural settlement to remove liquid supernatant; adding cleaning liquid, centrifuging and abandoning liquid supernatant; step 4, extracting the glass slide, fixing, dying and sealing. The preparation method has the effect of reducing the cell loss.

Description

technical field [0001] The invention relates to the field of cell detection, in particular to a liquid-based cell sheet preparation method. Background technique [0002] Liquid-based cytology examination is to use liquid-based thin-layer cytology detection system to detect cervical cells and carry out cytological classification diagnosis. Compared with the traditional cervical smear and Pap smear, liquid-based cytology has significantly improved the satisfaction of samples and the detection rate of abnormal cervical cells. Liquid-based cytology is currently a relatively advanced cervical cancer in the world. Cytology techniques. [0003] The Chinese patent with application publication number CN102680290A and application publication date of September 19, 2012 discloses a method for automatically producing liquid-based cell slides on a production device. The method includes: a. separating cells , use a cell breaker to repeatedly extract and discharge the cell fluid, and brea...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a liquid-based cell sheet production method, which solves the problem of cell loss in the separation process and has the effect of reducing cell loss

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