Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of preparation method of electrochemical malathion sensor

A malathion and sensor technology, applied in scientific instruments, instruments, and material analysis through electromagnetic means, can solve problems such as low yield and cumbersome process, and achieve low cost, simple preparation, and high selectivity detection Effect

Inactive Publication Date: 2019-03-12
UNIV OF JINAN
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, most of the synthesis methods are synthesized separately, and then compound the catalyst and the carrier, the process is cumbersome and the yield is not high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 FeMn-TiO 2 / MoS 2 preparation of

[0040] (1) Add 0.6 g of molybdenum disulfide powder, 0.2 mmol of iron salt and 0.2 mmol of manganese salt into 3 mL of n-butyllithium solution, and stir for 12 hours under nitrogen protection at 60 °C to obtain the reacted solution;

[0041] (2) Wash the reacted solution in step (1) with a non-polar solvent, and then perform ultrasonic treatment in a water bath at 60 °C. After the treatment, wash the treated solution with a non-polar solvent, and dry it in vacuum to obtain iron , Molybdenum disulfide nanomaterials co-intercalated with manganese;

[0042] (3) Add 500 mg of molybdenum disulfide nanomaterials co-intercalated with iron and manganese prepared in step (2) into 5 mL of tetrabutyl titanate, and after stirring for 1 hour, slowly add 0.5 mL of hydrofluoric acid while stirring acid, then reacted in a reactor at 160°C for 18 hours;

[0043] (4) The reaction product obtained in step (3) was centrifuged and washed thre...

Embodiment 2

[0049] Example 2 FeMn-TiO 2 / MoS 2 preparation of

[0050] (1) Add 0.6 g of molybdenum disulfide powder, 1.0 mmol of iron salt and 1.0 mmol of manganese salt into 5 mL of n-butyllithium solution, and stir for 24 hours under nitrogen protection at 30 °C to obtain the reacted solution;

[0051] (2) Wash the reacted solution in step (1) with a non-polar solvent, and then perform ultrasonic treatment in a water bath at 30 °C. After the treatment, wash the treated solution with a non-polar solvent, and dry it in vacuum to obtain iron , Molybdenum disulfide nanomaterials co-intercalated with manganese;

[0052] (3) Add 200 mg of molybdenum disulfide nanomaterials co-intercalated with iron and manganese prepared in step (2) into 5 mL of tetrabutyl titanate, stir for 1 hour, then slowly add 0.6 mL of hydrofluoric acid while stirring acid, then reacted in a reactor at 180°C for 20 hours;

[0053] (4) The reaction product obtained in step (3) was centrifuged and washed three times w...

Embodiment 3

[0059] Example 3 Preparation method of electrochemical malathion sensor

[0060] (1) Using the glassy carbon electrode as the working electrode, drop-coat 8 µL of FeMn-TiO on the surface of the electrode 2 / MoS 2 Sol, dry at room temperature;

[0061] (2) Wash the electrode obtained in step (1) with the buffer solution PBS, continue to drip-coat 8 µL of 10 µg / mL malathion antibody solution on the surface of the electrode, and store it in a refrigerator at 4 °C to dry;

[0062] (3) Wash the electrode obtained in step (2) with PBS, continue to drip-coat 8 µL of bovine serum albumin solution with a concentration of 100 µg / mL on the surface of the electrode, and store it in a refrigerator at 4 °C to dry;

[0063] (4) Wash the electrode obtained in step (3) with PBS, store and dry in a refrigerator at 4°C, and then prepare an electrochemical malathion sensor;

[0064] The FeMn-TiO 2 / MoS 2 The sol is 50 mg of FeMn-TiO 2 / MoS 2 The powder was dissolved in 10 mL ultrapure wate...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses a preparation method of an electrochemical sensor constructed on the basis of nano functional materials. The present invention firstly prepares a novel two-dimensional nano-electrode material——loaded bimetallic co-doped nanocomposite material FeMnTiO2 / MoS2, that is, iron and manganese co-doped titanium dioxide nano-squares in situ composite molybdenum disulfide two-dimensional Nanocomposite materials, using the good biocompatibility and large specific surface area of ​​the material, are loaded with malathion antibody. During detection, because iron and manganese co-doped titanium dioxide can catalyze hydrogen peroxide to generate O2 in situ, The electrochemical signal is generated, and then the specific quantitative binding of the antibody and the antigen affects the electron transport ability, so that the current intensity is correspondingly reduced, and finally the construction of a biosensor for the detection of malathion using a label-free electrochemical method is realized.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical sensor constructed on the basis of nano functional materials. It belongs to the technical field of new nanometer functional materials and biosensors. Background technique [0002] Malathion, also known as Malathion, is a drug insecticide suitable for controlling pests on crops such as tobacco, tea and mulberry. Malathion has good contact, stomach toxicity and certain fumigation effect, without systemic effect. In soil, malathion can slightly migrate to the deep soil layer through water leaching. Malathion in the soil can enter the plant through absorption by plant roots. After people eat such plants or plants containing their residues by mistake, malathion can enter the human body through the digestive tract, respiratory tract and intact skin and mucous membranes, causing nausea, vomiting, headache, diarrhea, general weakness and other poisoning Initial symptoms, long-term exposure...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 张勇邓保军杜斌王耀光吴丹
Owner UNIV OF JINAN