RNA nucleic acid for enhancing lung cancer radiotherapy sensitivity

A sensitive, nucleic acid technology, applied in the fields of molecular biology and genetic diagnostics, which can solve the role of miR-320a. Few studies, few, and no evidence that miR-320a enhances radiation resistance and sensitivity of lung adenocarcinoma to radiation response And other issues

Inactive Publication Date: 2016-11-23
BINZHOU MEDICAL COLLEGE
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] Before the present invention, there were not many research reports about miRNA enhancing radiation resistance of tumor cells, there were few studies on the role of miR-320a in cancer radiation resistance, and no miR-320a enhanced radiation resistance lung adenocarcinoma was sensitive to radiation. sex reports

Method used

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  • RNA nucleic acid for enhancing lung cancer radiotherapy sensitivity
  • RNA nucleic acid for enhancing lung cancer radiotherapy sensitivity
  • RNA nucleic acid for enhancing lung cancer radiotherapy sensitivity

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Experimental program
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Effect test

Embodiment Construction

[0016] Taking the designed miR-320a nucleic acid as an example, it shows that it significantly increases the sensitivity of radiation-resistant lung adenocarcinoma cells to radiotherapy, and improves the application of killing tumor cells.

[0017] 1. Required materials

[0018] A549 cells (Shanghai Institute of Cell Biology); A549 / R34 cells (A549 cells were obtained by accumulatively irradiating 68Gy with X-ray); RPMI-1640 medium (Gibco, Grand Island, New York, U.S.A.); fetal bovine serum (FBS, Gibco); Annexin V-FITC / PI (KeyGEN Biotech. Co. Ltd., Nanjing, China); MTT (0.5 mg / ml); Liposome 2000 (Invitrogen, Carlsbad, CA, USA).

[0019] 2. Cell culture and transfection

[0020] Lung adenocarcinoma A549 cells were cultured in 10% FBS RPMI-1640 medium at 37°C in an incubator with 5% CO2, and the cells were irradiated with 2Gy X-ray each time, once every 2 days, for a total of 34 times , with a total dose of 68Gy, to establish a radiation-resistant lung adenocarcinoma cell line ...

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Abstract

The invention belongs to the field of biotechnology and medical technology, and provides an application of self-designed RNA nucleic acid in enhancing the sensitivity of radiation-resistant lung cancer cells to radiation therapy. The RNA nucleic acid provided by the invention can significantly increase the sensitivity of radiation-resistant lung adenocarcinoma cells to radiation therapy by regulating the STAT3 signal, thereby providing a new idea for the treatment of radiation-resistant lung adenocarcinoma.

Description

technical field [0001] The invention belongs to the fields of molecular biology and gene diagnosis, and specifically relates to an application of self-designed RNA nucleic acid in increasing the sensitivity of radiation-resistant lung cancer cells to radiation therapy. Background technique [0002] Radiation therapy plays an important role in the treatment of non-small cell lung cancer, but the radiation resistance of lung cancer cells significantly affects the effect of radiation therapy. [0003] MicroRNA (miRNA) is a kind of small RNA with a length of about 20-22 nucleotides that does not encode proteins in eukaryotic genomes. miRNA is involved in the pathological process of cell proliferation, differentiation, apoptosis metabolism and tumorigenesis, and abnormal expression of miRNA is found in many human tumor cells. Studies have shown that as tumor suppressor genes (such as miR-15a or miR-16-1, etc.) or oncogenes (miR-155 or miR-17-92 gene cluster members), miRNA plays...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/713A61K48/00A61K51/00A61P35/00
CPCA61K31/713A61K51/00
Inventor 谢书阳王萍玉吕晴李有杰阎云飞
Owner BINZHOU MEDICAL COLLEGE
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