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Microbial fermentation device

A technology of microbial fermentation and reaction chamber, applied in the field of machinery to achieve the effect of reducing the impact

Inactive Publication Date: 2016-12-07
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the current equipment for cyanobacteria treatment is mainly suitable for the resource utilization of cyanobacteria, and is not suitable for providing suitable simulated samples undergoing aerobic and anaerobic microbial transformation processes for modern organic matter hydrocarbon generation evolution experiments.

Method used

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  • Microbial fermentation device

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Embodiment 1

[0023] The invention designs a small-scale simulated experimental device capable of degrading cyanobacterial organic matter through microbial aerobic and anaerobic degradation. This device can realize microbial aerobic and anaerobic degradation transformation of cyanobacteria organic matter, effectively produce humified organic matter, and can also collect gas products during the degradation process of cyanobacteria, providing suitable simulated samples for modern organic matter hydrocarbon generation evolution experiments , in order to carry out hydrocarbon generation simulation experiments close to geological reality.

[0024] like figure 1 Shown is a small-scale simulation experimental device for microbial degradation of cyanobacteria organic matter, which at least includes the following components, a reaction chamber 1 with a reaction chamber port, a reaction chamber cover 2 used in conjunction with the reaction chamber port, and the reaction chamber cover 2 is provided w...

Embodiment 2

[0039] A small-scale simulation experiment of microbial degradation of cyanobacterial organic matter

[0040] The working process of the small-scale simulation experiment device for microbial degradation of cyanobacteria organic matter is:

[0041] 1) Place the cyanobacteria sample collection in the reaction chamber 1, fill the cyanobacteria, and carry out cell disruption to the cyanobacteria (the crushing of the cyanobacteria can also be carried out before loading into the reaction chamber) to obtain the cyanobacteria fragmentation mixture. At this time, there is no need to plug Tighten the reaction chamber cover 2 made of polytetrafluoroethylene, install the stirrer 3 and then stir, and carry out the natural hydrolysis process according to the microbial transformation plan. During this process, there is no need to collect gas, therefore, at this time, the reaction chamber 1 and the first container 7 are not connected through the first conduit 4 temporarily.

[0042] 2) Inoc...

Embodiment 3

[0050] Small-scale simulation experiment of microbial degradation of various algae organic matter

[0051] 1) Hydrolysis stage: Put the fresh algae into the simulation device, use the temperature control device to control the temperature of the reaction chamber 1 to a constant temperature of 37°C, and hydrolyze for 7 days.

[0052] 2) Aerobic stage: inoculate the hydrolyzed algae with desulfurization bacteria, and desulfurize under aerobic conditions for 30 days. The microorganisms can convert sulfur into sulfate under aerobic conditions. and polythiosulfate to obtain energy.

[0053] 3) Anaerobic stage: the aerobic treated cyanobacteria were fed into argon to form an anaerobic environment, inoculated with nitrate-reducing bacteria for 7 days; then inoculated with sulfate-reducing bacteria for anaerobic treatment for 70 days, and the processed product was filtered Dry too much water, transfer to a freeze dryer, and dry to obtain humified organic matter that can be used to car...

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Abstract

The invention relates to the mechanical field and particularly relates to a microbial fermentation device. The microbial fermentation device comprises a reaction chamber, a stirrer, a first container and a first container cover, wherein the reaction chamber is provided with a reaction chamber hole and a reaction chamber cover matched with the reaction chamber hole for use; the stirrer is arranged in the reaction chamber; the first container is provided with a container hole; the first container cover is matched with the container hole, and a second through hole is formed in the container cover; and the reaction chamber and the first container are tightly connected through a first guide pipe, and two ends of the first guide pipe respectively penetrate through the first through hole and the second through hole, respectively.

Description

technical field [0001] The invention relates to the field of machinery, in particular to a device for microbial fermentation. Background technique [0002] Since the 1980s, our country has carried out research on algae hydrocarbon generation simulations. Since the early 1990s, the simulated algae have diversified, such as Acrylophytes, Coccolithophores, Panstar algae, unicellular algae, multicellular Sargassum, and marine algae. planktonic and benthic algae, etc. Through these simulation experiments, it is confirmed that bacteria and algae are the most important hydrocarbon-generating parent materials, and have the characteristics of hydrocarbon generation evolution such as early stage, multi-stage continuous hydrocarbon generation and multiple hydrocarbon generation peaks. However, the research on algae thermal simulation of hydrocarbon production mainly uses fresh algae as materials, and the hydrolysis and microbial degradation in the process of algae death and deposition...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/107C12M1/02
Inventor 腾格尔马亮帮刘文汇王杰陶成郑家锡
Owner CHINA PETROLEUM & CHEM CORP
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