QTL (Quantitative Trait Locus) linked molecular marker for controlling corn female parent haploidy production and application thereof

Abstract

The invention discloses a QTL (Quantitative Trait Locus) linked molecular marker for controlling corn female parent haploidy production. The molecular marker comprises a primer umc1295, wherein the sequence of the primer umc1295 is SEQ ID NO: F1:5'-GTCGATCTTCCTCCCCATCA-3'; SEQ ID NO: R1: 5'-CGTTTCTATCTATGGAGGAGTGCG-3'. Through SSR molecular marker and QTL analysis, a QTL locus for controlling the corn female parent haploidy production exists on a seventh chromosome, is linked with the molecular marker umc1295, and is located on 7.04bin of the seventh chromosome. The analysis result indicates that whether the marker exists or not directly relates to the haploidy production capacity of plants, and the marker can be used for forecasting female parent haploidy production frequency. Compared with the prior art, through the detection of the molecular marker, the haploidy production capacity of corn female parent can be forecasted in an early stage, so that the blindness of induction is reduced, the induction efficiency is improved, and the engineering application of a haploidy breeding technique is accelerated.

Description

technical field [0001] The invention belongs to the technical fields of maize genetic breeding and molecular biology, and in particular relates to a molecular marker for controlling the generation of QTL linkage of maize maternal haploid and its application. Background technique [0002] The core of maize breeding lies in the selection of excellent inbred lines. The conventional method of inbred line selection requires 6-8 generations of continuous selfing, which has a long breeding cycle and high cost; while haploid breeding technology only needs 2 generations. Obtaining homozygous inbred lines shortens the breeding cycle, saves breeding costs, and improves breeding efficiency. Together with molecular marker-assisted breeding technology and transgenic technology, it has become the three core technologies of modern maize breeding. The generation of haploid is the prerequisite for the application of haploid breeding technology. At present, the research on haploid generation i...

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Problems solved by technology

[0003] In terms of the influence of germplasm of different heterogeneous groups on the haploid induction rate, Li Guoliang et al. (2008) used Zhongnong Dagaoyu No. 1 to induce 5 different germplasm groups of maize, and the results showed that there were significant differences among different germplasm groups , the Lancaster group and the Reid group were higher, respectively 4.92% and 4.78%, followed by the Tangsipingtou and Luda Hongbone groups, with a haploid frequency of about 4%, while the warm germplasm group produced The ability of haploid is the lowest, only 2.04%, which is significantly lower than the other four germplasm groups; Cai Quan et al. (2012) studied that the induction rate of the same induction line to different genetic basic materials is also different, and the induction rate of the general Rhett blood Higher; Sun Rui et al. (2013) used Gaoyu No. 1 and Gaoyu No. 5 to induce the inbred lines of the four germplasm groups. Among them, the haploid induction rate of Luda red bone and Lancaster taxa is higher, and that of Reid and Tang Sipingtou taxa is lower

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