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A preparation method of Toltrazuril molecularly imprinted electrochemical sensor

A technology of toltrazuril and molecular imprinting, which is applied in the field of materials, can solve the problems of difficult elution of template molecules, easy peeling off of the coating, and poor stability, and achieve the effects of fast electron transmission, firm coating, and high stability

Inactive Publication Date: 2018-08-14
GUANGDONG FOOD & DRUG VOCATIONAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of the molecularly imprinted sensor prepared by the sol-gel method, the coating is easy to fall off, crack, the template molecules are not easy to elute, and the stability is poor. The preparation method, the prepared toltrazuril molecularly imprinted electrochemical sensor method is simple and convenient, the template molecules are easy to elute, the coating is firm, the result is stable, the reproducibility is good, the selectivity is high, and it is sensitive, and can be applied to food and feed properly. Determination of Residues of Trajuzil

Method used

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  • A preparation method of Toltrazuril molecularly imprinted electrochemical sensor
  • A preparation method of Toltrazuril molecularly imprinted electrochemical sensor
  • A preparation method of Toltrazuril molecularly imprinted electrochemical sensor

Examples

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Effect test

Embodiment 1

[0027] Example 1: Preparation of Toltrazuril molecularly imprinted electrochemical sensor according to the present invention

[0028] The schematic diagram of the preparation of Toltrazuril molecularly imprinted electrochemical sensor of the present invention is as follows figure 1 Shown, preparation method comprises the following steps:

[0029] (1) Put 0.5g of β-cyclodextrin (β-CD) in a 25mL round bottom flask, add 3mL of 4mol·L -1 NaOH solution, stirred to dissolve completely, alkalized in water bath at 35°C for 1h. 1mL0.50g·mL -1 Chloroacetic acid aqueous solution was slowly dropped dropwise into a round bottom flask, and reacted in a water bath at 60°C for 4h. Adjust the pH value to neutral with glacial acetic acid, add methanol to extract, filter, and dry at 70°C to obtain carboxylated cyclodextrin.

[0030] (2) Dissolve 0.30 g of carboxymethyl-β-CD and 0.054 g of Toltrazuril in a mixed solvent of 6 mL of water and 2 mL of absolute ethanol, ultrasonically disperse f...

Embodiment 2

[0034] Example 2: Preparation of Toltrazuril molecularly imprinted electrochemical sensor according to the present invention

[0035] (1) Put 0.65g of β-cyclodextrin (β-CD) in a 25mL round bottom flask, add 3mL of 5 mol·L -1 NaOH solution, stirred to dissolve it completely, alkalized in water bath at 40°C for 1h. 1mL 0.60g·mL -1 Chloroacetic acid aqueous solution was slowly dropped dropwise into a round-bottomed flask, reacted in a water bath at 65°C for 4 hours, then adjusted the pH value to 7 with glacial acetic acid, extracted with methanol, filtered, and dried at 75°C to obtain carboxylated cyclopaste Refined.

[0036] (2) Dissolve 0.15g of carboxymethyl-β-CD and 0.027g of Toltrazuril in a mixed solvent of 3mL of water and 1mL of absolute ethanol, ultrasonically disperse for 0.5h, seal, and stir at 30°C for 1h for pre-polymerization. Add 423 μL tetraethyl orthosilicate, add 100 μL glacial acetic acid after purging nitrogen for 15 minutes, seal, and react in a water bath...

Embodiment 3

[0040] Example 3: Preparation of Toltrazuril molecularly imprinted electrochemical sensor according to the present invention

[0041] (1) Put 0.8g of β-cyclodextrin (β-CD) in a 25mL round bottom flask, add 3mL of 6mol·L -1 NaOH solution, stirred to dissolve it completely, alkalized in water bath at 45°C for 1h. 1mL0.70g·mL -1 Chloroacetic acid aqueous solution was slowly dropped dropwise into a round-bottomed flask, reacted in a water bath at 70°C for 4 hours, then adjusted the pH value to neutral with glacial acetic acid, extracted with methanol, filtered, and dried at 80°C to obtain carboxylated ring dextrin.

[0042] (2) Dissolve 0.60g of carboxymethyl-β-CD and 0.107g of Toltrazuril in a mixed solvent of 12mL of water and 4mL of absolute ethanol, ultrasonically disperse for 0.5h, seal, and stir at 30°C for 1h for pre-polymerization. Add 1.7 mL tetraethyl orthosilicate, add 400 μL glacial acetic acid after nitrogen gas for 15 minutes, seal, and react in water bath for 12...

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Abstract

The invention relates to a preparation method of a toltrazuril molecular imprinting electrochemical sensor. The preparation method comprises the following steps: taking toltrazuril as the template molecule, carboxylated beta-cyclodextrin as the functional monomer, and tetraethyl ortho-silicate as the crosslinking agent, carrying out reactions under an acidic condition to obtain a toltrazuril sol imprinted polymer; dropwise painting a reduced graphene dispersion liquid on a platinum electrode (phi 2mm), drying the platinum electrode under an infrared lamp; then dropwise painting an aminated titanium dioxide dispersion liquid on the platinum electrode, drying the platinum electrode under an infrared lamp; then dropwise painting the toltrazuril sol imprinted polymer on the platinum electrode, drying the platinum electrode at a room temperature; placing the platinum electrode in a HCl solution, and washing off the template molecules in a thermostatic water bath to obtain a toltrazuril molecular imprinting electrochemical sensor. The operation is simple, the coating is firm, the template molecules can be easily washed out, and the prepared molecular printing electrochemical sensor has the advantages of stability, good repeatability, high sensitivity, and good selectivity, and can be used to detect toltrazuril in food and feed.

Description

technical field [0001] The invention belongs to the technical field of materials, and relates to a preparation method of a molecularly imprinted electrochemical sensor, in particular to a preparation method of a toltrazuril molecularly imprinted electrochemical sensor, which can be applied to the detection of toltrazuril in food and feed samples. Background technique [0002] Toltrazuril (Toltrazuril), also known as methyl triazone, is a triazinone anticoccidial drug. It can effectively treat coccidiosis outbreaks in poultry, rabbits, piglets and other animals, so it is widely used. The main metabolites of Toltrazuril are Toltrazuril Alum and Toltrazuril Alum. Toxicology tests show that Toltrazuril may have teratogenic toxicity. The maximum residue limit in meat products stipulated by the European Union is 100-600μg·kg -1 , animals that lay eggs (for consumption) are not allowed (Jong-Hwan LIM, etal. J. Vet. Med. Sci., 2010, 72 (8): 1085-1087; Committee for medicinal product...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30
CPCG01N27/30
Inventor 利健文夏黎崔丽京陈任宏汪小根
Owner GUANGDONG FOOD & DRUG VOCATIONAL COLLEGE
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